Detecting and Removing Data Artifacts in Hadamard Transform Ion Mobility-Mass Spectrometry Measurements

Spencer A Prost; Kevin L Crowell; Erin S Baker; Yehia M Ibrahim; Brian H Clowers; Matthew E Monroe; Gordon A Anderson; Richard D Smith; Samuel H Payne

doi:10.1007/s13361-014-0895-y

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Detecting and Removing Data Artifacts in Hadamard Transform Ion Mobility-Mass Spectrometry Measurements

Journal article

Open access

Detecting and Removing Data Artifacts in Hadamard Transform Ion Mobility-Mass Spectrometry Measurements

Spencer A Prost, Kevin L Crowell, Erin S Baker, Yehia M Ibrahim, Brian H Clowers, Matthew E Monroe, Gordon A Anderson, Richard D Smith and Samuel H Payne

Journal of the American Society for Mass Spectrometry, Vol.25(12), pp.2020-2027

12/2014

DOI: https://doi.org/10.1007/s13361-014-0895-y

Handle:

https://hdl.handle.net/2376/103924

PMID: 24796262

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https://doi.org/10.1007/s13361-014-0895-yView

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Abstract

Computational proteomics

Sensitivity

Data analysis

Ion mobility

Hadamard transform

Bioinformatics

Applying Hadamard transform multiplexing to ion mobility separations (IMS) can significantly improve the signal-to-noise ratio and throughput for IMS coupled mass spectrometry (MS) measurements by increasing the ion utilization efficiency. However, it has been determined that fluctuations in ion intensity as well as spatial shifts in the multiplexed data lower the signal-to-noise ratios and appear as noise in downstream processing of the data. To address this problem, we have developed a novel algorithm that discovers and eliminates data artifacts. The algorithm employs an analytical approach to identify and remove artifacts from the data, decreasing the likelihood of false identifications in subsequent data processing. Following application of the algorithm, IMS-MS measurement sensitivity is greatly increased and artifacts that previously limited the utility of applying the Hadamard transform to IMS are avoided.

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Title: Detecting and Removing Data Artifacts in Hadamard Transform Ion Mobility-Mass Spectrometry Measurements
Creators: Spencer A Prost - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Kevin L Crowell - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Erin S Baker - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Yehia M Ibrahim - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Brian H Clowers - Department of Chemistry, Washington State University, Pullman, WA 99164, USA
Matthew E Monroe - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Gordon A Anderson - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Richard D Smith - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Samuel H Payne - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Publication Details: Journal of the American Society for Mass Spectrometry, Vol.25(12), pp.2020-2027
Academic Unit: Chemistry, Department of
Identifiers: 99900546516201842
Language: English
Resource Type: Journal article