Journal article
Detection and mapping of quantitative trait loci that determine responsiveness of mice to nitrous oxide antinociception
Neuroscience, Vol.123(3), pp.743-749
2004
Handle:
https://hdl.handle.net/2376/115079
PMID: 14706786
Abstract
Exposure to 70% N
2O evokes a robust antinociceptive effect in C57BL/6 (B6) but not in DBA/2 (D2) inbred mice. This study was conducted to identify quantitative trait loci (QTL) in the mouse genome that might determine responsiveness to N
2O. Offspring from the F
2 generation bred from B6 and D2 progenitors exhibited a broad range of responsiveness to N
2O antinociception as determined by the acetic acid-induced abdominal constriction test. QTL analysis was then used to dissect this continuous trait distribution into component loci, and to map them to broad chromosomal regions. To this end, 24 spleens were collected from each of the following four groups: male and female F
2 mice responding to 70% N
2O in oxygen with 100% response (high-responders); and male and female F
2 mice responding with 0% response (low-responders). Genomic DNA was extracted from the spleens and genotyped with simple sequence length polymorphism MapPairs markers. Findings were combined with findings from the earlier QTL analysis from BXD recombinant inbred mice [Brain Res 725 (1996) 23]. Combined results revealed two significant QTL that influence responsiveness to nitrous oxide on proximal chromosome 2 and distal chromosome 5, and one suggestive QTL on midchromosome 18. The chromosome 2 QTL was evident only in males. A significant interaction was found between a locus on chromosome 6 and another on chromosome 13 with a substantial effect on N
2O antinociception.
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Details
- Title
- Detection and mapping of quantitative trait loci that determine responsiveness of mice to nitrous oxide antinociception
- Creators
- J.L Mueller - Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107, USAE.A Ellenberger - Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107, USAL.K Vaughn - Department of Biomedical Sciences, Marquette University, Milwaukee, WI 53201, USAJ.K Belknap - Research Service (R&D5), Portland VA Medical Center, and Department of Behavioral Neuroscience, Oregon Health and Science University, Portland, OR 97239, USAR.M Quock - Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107, USA
- Publication Details
- Neuroscience, Vol.123(3), pp.743-749
- Academic Unit
- Psychology, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547515801842
- Language
- English
- Resource Type
- Journal article