Journal article
Detection by PCR of Neospora caninum in Fetal Tissues from Spontaneous Bovine Abortions
Journal of clinical microbiology, Vol.37(12), pp.4059-4064
12/1999
Handle:
https://hdl.handle.net/2376/109053
PMCID: PMC85881
PMID: 10565932
Abstract
The routine diagnosis of
Neospora caninum
abortion is based upon histopathologic changes in fetal tissues and identification of tissue parasites by immunohistochemistry. Confirmation of
N. caninum
infection by immunohistochemistry has low sensitivity. In the present study, we examined the utility of PCR in detecting
N. caninum
infection in fetal tissues from spontaneous bovine abortion. DNA was obtained from fresh and formalin-fixed tissues from 61 bovine fetuses submitted for abortion diagnosis. Histopathology and immunohistochemistry determined the true status of
N. caninum
infection in each fetus. In formalin-fixed paraffin-embedded tissues, PCR detected
N. caninum
DNA in 13 of 13 true-positive fetuses (100%) and in 1 of 16 true-negative fetuses (6%). In fresh or frozen tissues, PCR detected
N. caninum
DNA in 10 of 13 true-positive fetuses (77%) and 0 of 11 true-negative fetuses (0%). PCR also detected
N. caninum
DNA in 6 of 8 fetuses that had typical lesions of
N. caninum
but were immunohistochemistry negative, indicating a higher sensitivity of PCR in comparison to that of immunohistochemistry.
N. caninum
DNA was amplified most consistently from brain tissue. PCR detection of
N. caninum
DNA in formalin-fixed, paraffin-embedded tissues was superior to that in fresh tissues, presumably because of the increased accuracy of sample selection inherent in histologic specimens.
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Details
- Title
- Detection by PCR of Neospora caninum in Fetal Tissues from Spontaneous Bovine Abortions
- Creators
- Timothy V Baszler - Department of Veterinary Microbiology and PathologyLawrence J. C Gay - Department of Veterinary Microbiology and PathologyMaureen T Long - Department of Veterinary Microbiology and PathologyBruce A Mathison - Department of Veterinary Microbiology and Pathology
- Publication Details
- Journal of clinical microbiology, Vol.37(12), pp.4059-4064
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547230701842
- Language
- English
- Resource Type
- Journal article