Journal article
Detection of Anaplasma marginale rickettsemia prior to onset of clinical signs by using an antigen capture enzyme-linked immunosorbent assay
Journal of clinical microbiology, Vol.29(7), pp.1542-1544
07/1991
Handle:
https://hdl.handle.net/2376/116830
PMCID: PMC270153
PMID: 1885753
Abstract
An antigen capture enzyme-linked immunosorbent assay was developed by using monoclonal antibodies to conserved epitopes on the Anaplasma marginale MSP1a surface protein. The assay sensitivity was 1.1 (+/- 0.5)% parasitized erythrocytes, and all infected cattle were detected prior to development of 2.0%-parasitized erythrocytes. Positive tests preceded the onset of anemia by a mean of 2 days. The assay was specific for anaplasmosis, as demonstrated by nonreactivity with another common hemoparasitic pathogens.
Metrics
9 Record Views
Details
- Title
- Detection of Anaplasma marginale rickettsemia prior to onset of clinical signs by using an antigen capture enzyme-linked immunosorbent assay
- Creators
- E S Trueblood - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040T C McGuire - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040G H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040
- Publication Details
- Journal of clinical microbiology, Vol.29(7), pp.1542-1544
- Identifiers
- 99900548273601842
- Language
- English
- Resource Type
- Journal article