Journal article
Detection of Anaplasma ovis infection in goats by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay
Journal of clinical microbiology, Vol.33(3), pp.675-679
03/1995
Handle:
https://hdl.handle.net/2376/117284
PMCID: PMC228012
PMID: 7538510
Abstract
A competitive inhibition enzyme-linked immunosorbent assay (ELISA) based on a major surface protein 5 (MSP5) B-cell epitope conserved among Anaplasma species was used to detect goats infected with Anaplasma ovis. We examined strains of A. ovis isolated from goats in Kenya and demonstrated that MSP5 and the target B-cell epitope, bound by monoclonal antibody ANAF16C1, were conserved. Sera from 149 goats in four regions of Kenya and from 302 goats in six U.S. states were tested for the presence of epitope-specific antibodies with the MSP5 competitive inhibition ELISA. Evidence that the assay can be used to detect A. ovis-infected goats includes the following: (i) 53 goats raised in confinement with arthropod control were all seronegative; (ii) six goats experimentally infected with A. ovis seroconverted at the same time that they developed detectable rickettsemia; (iii) seroconverted goats remained seropositive, consistent with the persistence of A. ovis in goats and the presence of anti-MSP5 antibody in cattle persistently infected with Anaplasma marginale; and (iv) 119 of 127 known A. ovis-infected goats in Kenya were seropositive. A. ovis infection, as determined serologically and by demonstration of infected erythrocytes, in goats from the four regions in Kenya was highly prevalent. In contrast, despite the presence of A. ovis and competent arthropod vectors in the United States, the prevalence of infection appeared to be very low. The high prevalence in Kenya and the occurrence of anemia in persistently infected goats may be impediments to current efforts to increase milk yields on small farms.
Metrics
12 Record Views
Details
- Title
- Detection of Anaplasma ovis infection in goats by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay
- Creators
- L W Ndung'u - Veterinary Research Center, Kenya Agricultural Research Institute, KabeteC Aguirre - Veterinary Research Center, Kenya Agricultural Research Institute, KabeteF R Rurangirwa - Veterinary Research Center, Kenya Agricultural Research Institute, KabeteT F McElwain - Veterinary Research Center, Kenya Agricultural Research Institute, KabeteT C McGuire - Veterinary Research Center, Kenya Agricultural Research Institute, KabeteD P Knowles - Veterinary Research Center, Kenya Agricultural Research Institute, KabeteG H Palmer - Veterinary Research Center, Kenya Agricultural Research Institute, Kabete
- Publication Details
- Journal of clinical microbiology, Vol.33(3), pp.675-679
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Identifiers
- 99900548359201842
- Language
- English
- Resource Type
- Journal article