Journal article
Detection of Cattle Naturally Infected with Anaplasma marginale in a Region of Endemicity by Nested PCR and a Competitive Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5
Journal of clinical microbiology, Vol.36(3), pp.777-782
03/1998
Handle:
https://hdl.handle.net/2376/103315
PMCID: PMC104624
PMID: 9508311
Abstract
A competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5 (rMSP5-cELISA) of
Anaplasma marginale
was validated in a naturally infected cattle herd in an area of eastern Oregon where
A. marginale
is endemic. The true positive and negative
A. marginale
infection status of 235 randomly selected cattle was determined by using a nested PCR (nPCR) coupled with
msp5
sequence analysis and hybridization. Judgment of the reliability of the nPCR and hybridization for detection of persistent infections was based on three observations. First, the nPCR was able to detect as few as 30 infected erythrocytes per ml. Second, the nPCR was able to consistently detect low levels of rickettsemia in seven carrier cattle experimentally infected with
A. marginale
. Third,
msp5
sequence analysis showed >95% identity among 30 nPCR amplicons from cattle naturally infected with field strains of
A. marginale
. The nPCR and hybridization identified 151 infected and 84 uninfected cattle among the 235 animals tested. With a cutoff point of 28%, the rMSP5-cELISA showed a sensitivity of 96% and a specificity of 95%. These results indicate that the rMSP5-cELISA can sensitively and specifically detect cattle with naturally acquired persistent
A. marginale
infections and suggest that it is an excellent assay for epidemiological studies, eradication programs, and regulation of international cattle movement.
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Details
- Title
- Detection of Cattle Naturally Infected with Anaplasma marginale in a Region of Endemicity by Nested PCR and a Competitive Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5
- Creators
- Susana Torioni de Echaide - Instituto Nacional de Tecnología Agropecuaria, EEA Rafaela, Rafaela, Santa Fe, ArgentinaDonald P Knowles - Instituto Nacional de Tecnología Agropecuaria, EEA Rafaela, Rafaela, Santa Fe, ArgentinaTravis C McGuire - Instituto Nacional de Tecnología Agropecuaria, EEA Rafaela, Rafaela, Santa Fe, ArgentinaGuy H Palmer - Instituto Nacional de Tecnología Agropecuaria, EEA Rafaela, Rafaela, Santa Fe, ArgentinaCarlos E Suarez - Instituto Nacional de Tecnología Agropecuaria, EEA Rafaela, Rafaela, Santa Fe, ArgentinaTerry F McElwain - Instituto Nacional de Tecnología Agropecuaria, EEA Rafaela, Rafaela, Santa Fe, Argentina
- Publication Details
- Journal of clinical microbiology, Vol.36(3), pp.777-782
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546517401842
- Language
- English
- Resource Type
- Journal article