Journal article
Detection of Mycobacterium bovis infection in cattle using an immunoassay for bovine soluble interleukin-2 receptor-α (sIL-2R-α) produced by peripheral blood T-lymphocytes following incubation with tuberculin PPD
Veterinary immunology and immunopathology, Vol.56(1), pp.65-76
1997
Handle:
https://hdl.handle.net/2376/114642
PMID: 9220581
Abstract
After activation of T-lymphocytes with antigen there is an increase in the expression of interleukin-2 receptor-α (IL-2R-α) followed by the release of a soluble form of the molecule (sIL-2R-α) from the membrane of the stimulated cells. The present study investigates the novel use of the release of sIL-2R-α from activated T-lymphocytes as a marker of cell-mediated immunity (CMI) in cattle infected with
Mycobacterium bovis. An enzyme immunoassay was used to detect sIL-2R-α produced following incubation of bovine peripheral blood mononuclear cells with mycobacterial antigens. Using this assay,
63
67
cattle naturally infected with
M. bovis were identified whereas only
1
51
uninfected animals were considered to give a positive result. This assay is more convenient to use than lymphocyte proliferation assays which involve the use of radionucleosides. It should prove useful for monitoring the immunological activation of bovine T-lymphocytes in a variety of situations including the development of CMI responses in cattle to novel mycobacterial antigens or potential vaccines.
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Details
- Title
- Detection of Mycobacterium bovis infection in cattle using an immunoassay for bovine soluble interleukin-2 receptor-α (sIL-2R-α) produced by peripheral blood T-lymphocytes following incubation with tuberculin PPD
- Creators
- E.M ÓNualláin - Department of Large Animal Clinical Studies, Faculty of Veterinary Medicine, University College Dublin, Ballsbridge, Dublin 4, IrelandW.C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USAE Costello - Department of Agriculture, Food and Forestry, Veterinary Research Laboratory, Abbottstown, Castleknock, Dublin 15, IrelandJ.M Pollock - Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, UKM.L Monaghan - Department of Large Animal Clinical Studies, Faculty of Veterinary Medicine, University College Dublin, Ballsbridge, Dublin 4, Ireland
- Publication Details
- Veterinary immunology and immunopathology, Vol.56(1), pp.65-76
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547827001842
- Language
- English
- Resource Type
- Journal article