Journal article
Detection of antibodies to a recombinant Cryptosporidium parvum P23 in serum and feces from neonatal calves
The Journal of parasitology, Vol.89(5), pp.918-923
10/01/2003
Handle:
https://hdl.handle.net/2376/106340
Abstract
Passive transfer of maternal antibodies via colostrum is important to protect newborn ruminants against microbial pathogens. In this study, 10 sets of calf serum, a sample of the colostrum fed to the calf, and serial fecal samples through the first 6 days after birth were collected from arbitrarily selected newborn Holstein heifers. A recombinant Cryptosporidium parvum p23, termed rC7, was used to determine whether anti-C. parvum antibodies can be detected in clinically normal neonates. The results demonstrated that serum, the associated colostrum, and fecal samples contained anti-rC7 antibodies. IgM and IgG1 anti-rC7 tended to be present in highest titers. The presence of specific antibodies to C. parvum was confirmed using Western blots of purified sporozoite membranes probed with serum and colostral whey. Collectively, the results indicated that neonatal calves had antibodies to C. parvum as early as 1 day after birth and suggested that the antibodies were passively transferred.
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Details
- Title
- Detection of antibodies to a recombinant Cryptosporidium parvum P23 in serum and feces from neonatal calves
- Creators
- H WangJ B SwainT E BesserD JasmerC R Wyatt
- Publication Details
- The Journal of parasitology, Vol.89(5), pp.918-923
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Identifiers
- 99900546940801842
- Language
- English
- Resource Type
- Journal article