Journal article
Detection of organophosphate pesticide using polyaniline and carbon nanotubes composite based on acetylcholinesterase inhibition
Journal of nanoscience and nanotechnology, Vol.10(9), pp.5662-5666
09/2010
Handle:
https://hdl.handle.net/2376/114546
PMID: 21133088
Abstract
Acetylcholinesterase (AChE) activity may be useful biomarker for detecting of organophosphate pesticides (OP). Thus a sensitive biosensor for quantitative determination of OP based on AChE biomonitoring was developed. Multi-walled carbon nanotube (MWCNT)/polyaniline (PANI) composite film was prepared by electrochemical polymerization. The immobilized AChE catalyzed the hydrolysis of acetylthiocholine chloride to produce thiocholine, which engendered an irreversible oxidation peak. The enzyme activity was monitored by measuring the oxidation current of thiocholine and further detection of OP. The developed sensor provided a new promising tool for pesticide analysis and assay of enzyme activity.
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Details
- Title
- Detection of organophosphate pesticide using polyaniline and carbon nanotubes composite based on acetylcholinesterase inhibition
- Creators
- Daiqin Chen - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR ChinaChuanhuizi ChenDan Du
- Publication Details
- Journal of nanoscience and nanotechnology, Vol.10(9), pp.5662-5666
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- United States
- Identifiers
- 99900547515501842
- Language
- English
- Resource Type
- Journal article