Journal article
Detection of ovine herpesvirus 2 major capsid gene transcripts as an indicator of virus replication in shedding sheep and clinically affected animals
Virus research, Vol.132(1), pp.69-75
2008
Handle:
https://hdl.handle.net/2376/107485
PMID: 18054405
Abstract
The aim of this study was to identify tissues where ovine herpesvirus 2 (OvHV-2) replication occurs in vivo. A reverse-transcriptase PCR targeting the OvHV-2 major capsid protein gene (ORF 25) was developed and the presence of transcripts used as an indicator of virus replication in naturally infected sheep, and cattle and bison with sheep-associated malignant catarrhal fever (SA-MCF). ORF 25 transcripts were detected in 18 of 60 (30%) turbinate, trachea, and lung samples from five sheep experiencing a shedding episode; 12 of the 18 positive samples were turbinates. ORF 25 transcripts were not detected in any other tissue from the shedding sheep (
n
=
55). In contrast, 86 of 102 (84%) samples from clinically affected bovine and bison tissues, including brain, kidney, intestine, and bladder, had ORF 25 transcripts. The data strongly suggest that OvHV-2 replication is localized to the respiratory tract of shedding sheep, predominantly in the turbinate, while it occurs in virtually all tissues of cattle and bison with SA-MCF. These findings represent an important initial step in understanding viral pathogenesis, and in potentially establishing a system for OvHV-2 propagation in vitro.
Metrics
11 Record Views
Details
- Title
- Detection of ovine herpesvirus 2 major capsid gene transcripts as an indicator of virus replication in shedding sheep and clinically affected animals
- Creators
- Cristina W Cunha - Animal Diseases Research Unit, Agricultural Research Service, United States Department of Agriculture, Washington State University, P.O. Box 646630, Pullman, WA 99164-6630, USADonald L Traul - Animal Diseases Research Unit, Agricultural Research Service, United States Department of Agriculture, Washington State University, P.O. Box 646630, Pullman, WA 99164-6630, USANaomi S Taus - Animal Diseases Research Unit, Agricultural Research Service, United States Department of Agriculture, Washington State University, P.O. Box 646630, Pullman, WA 99164-6630, USAJ. Lindsay Oaks - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USADonal O’Toole - Wyoming State Veterinary Laboratory, University of Wyoming, Laramie, WY 82070, USAChristine M Davitt - Franceschi Microscopy and Imaging Center, Washington State University, Pullman, WA 99164-4210, USAHong Li - Animal Diseases Research Unit, Agricultural Research Service, United States Department of Agriculture, Washington State University, P.O. Box 646630, Pullman, WA 99164-6630, USA
- Publication Details
- Virus research, Vol.132(1), pp.69-75
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547637001842
- Language
- English
- Resource Type
- Journal article