Detection of protein S-sulfhydration by a tag-switch technique

Dehui Zhang; Igor Macinkovic; Nelmi O Devarie-Baez; Jia Pan; Chung-Min Park; Kate S Carroll; Milos R Filipovic; Ming Xian

doi:10.1002/anie.201305876

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Detection of protein S-sulfhydration by a tag-switch technique

Journal article

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Peer reviewed

Detection of protein S-sulfhydration by a tag-switch technique

Dehui Zhang, Igor Macinkovic, Nelmi O Devarie-Baez, Jia Pan, Chung-Min Park, Kate S Carroll, Milos R Filipovic and Ming Xian

Angewandte Chemie (International ed.), Vol.53(2), pp.575-581

01/07/2014

DOI: https://doi.org/10.1002/anie.201305876

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https://hdl.handle.net/2376/106976

PMCID: PMC4306352

PMID: 24288186

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Abstract

Biotin - chemistry

Human Umbilical Vein Endothelial Cells

Glutathione Peroxidase - chemistry

Jurkat Cells

Humans

Hydrogen Sulfide - chemistry

Sulfides - chemistry

Serum Albumin, Bovine - chemistry

Sulfhydryl Compounds - chemistry

Protein Processing, Post-Translational

Cysteine - chemistry

Microscopy, Fluorescence

Protein S-sulfhydration (forming -S-SH adducts from cysteine residues) is a newly defined oxidative posttranslational modification and plays an important role in H2 S-mediated signaling pathways. In this study we report the first selective, "tag-switch" method which can directly label protein S-sulfhydrated residues by forming stable thioether conjugates. Furthermore we demonstrate that H2 S alone cannot lead to S-sulfhydration and that the two possible physiological mechanisms include reaction with protein sulfenic acids (P-SOH) or the involvement of metal centers which would facilitate the oxidation of H2 S to HS(.) .

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Title: Detection of protein S-sulfhydration by a tag-switch technique
Creators: Dehui Zhang - Department of Chemistry, Washington State University, Pullman, WA 99164 (USA)
Igor Macinkovic
Nelmi O Devarie-Baez
Jia Pan
Chung-Min Park
Kate S Carroll
Milos R Filipovic
Ming Xian
Publication Details: Angewandte Chemie (International ed.), Vol.53(2), pp.575-581
Academic Unit: Chemistry, Department of
Publisher: Germany
Grant note: R01 GM102187 / NIGMS NIH HHS R01 CA174864 / NCI NIH HHS
Identifiers: 99900546866101842
Language: English
Resource Type: Journal article