Journal article
Development and Initial Characterization of a HAPPY Panel for Mapping the X. Tropicalis Genome
International journal of biological sciences, Vol.7(7), pp.1037-1044
2011
Handle:
https://hdl.handle.net/2376/106536
PMCID: PMC3164153
PMID: 21912511
Abstract
HAPPY mapping was designed to pursue the analysis of approximately random
HAP
loid DNA breakage samples using the
P
ol
Y
merase chain reaction for mapping genomes. In the present study, we improved the method and integrated two other molecular techniques into the process: whole genome amplification and the Sequenom SNP (single nucleotide polymorphism) genotyping assay in order to facilitate whole genome mapping of
X. tropicalis
. The former technique amplified enough DNA materials to genotype a large number of markers, while the latter allowed for relatively high throughput marker genotyping with multiplex assays on the HAPPY lines. A total of 58
X. tropicalis
genes were genotyped on an initial panel of 383 HAPPY lines, which contributed to formation of a working panel of 146 lines. Further genotyping of 29 markers on the working panel led to construction of a HAPPY map for the
X. tropicalis
genome. We believe that our improved HAPPY method described in the present study has paved the way for the community to map different genomes with a simple, but powerful approach.
Metrics
2 Record Views
Details
- Title
- Development and Initial Characterization of a HAPPY Panel for Mapping the X. Tropicalis Genome
- Creators
- Zhihua Jiang - 1. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAJennifer J Michal - 1. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAKenneth B Beckman - 2. Biomedical Genomics Center, University of Minnesota, Minneapolis, MN 55455, USAJessica B Lyons - 3. Department of Molecular & Cell Biology, University of California Berkeley, Berkeley, CA 94720-3200, USAMing Zhang - 1. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAZengxiang Pan - 1. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USADaniel S Rokhsar - 3. Department of Molecular & Cell Biology, University of California Berkeley, Berkeley, CA 94720-3200, USARichard M Harland - 3. Department of Molecular & Cell Biology, University of California Berkeley, Berkeley, CA 94720-3200, USA
- Publication Details
- International journal of biological sciences, Vol.7(7), pp.1037-1044
- Academic Unit
- Animal Sciences, Department of
- Publisher
- Ivyspring International Publisher; Sydney
- Identifiers
- 99900546861101842
- Language
- English
- Resource Type
- Journal article