Journal article
Development and characterization of 37 novel EST-SSR markers in Pisum sativum (Fabaceae)
Applications in plant sciences, Vol.1(1), pp.1200249-n/a
01/2013
Handle:
https://hdl.handle.net/2376/109786
PMID: 25202482
Abstract
Simple sequence repeat markers were developed based on expressed sequence tags (EST-SSR) and screened for polymorphism among 23 Pisum sativum individuals to assist development and refinement of pea linkage maps. In particular, the SSR markers were developed to assist in mapping of white mold disease resistance quantitative trait loci. •
Primer pairs were designed for 46 SSRs identified in EST contiguous sequences assembled from a 454 pyrosequenced transcriptome of the pea cultivar, 'LIFTER'. Thirty-seven SSR markers amplified PCR products, of which 11 (30%) SSR markers produced polymorphism in 23 individuals, including parents of recombinant inbred lines, with two to four alleles. The observed and expected heterozygosities ranged from 0 to 0.43 and from 0.31 to 0.83, respectively. •
These EST-SSR markers for pea will be useful for refinement of pea linkage maps, and will likely be useful for comparative mapping of pea and as tools for marker-based pea breeding.
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Details
- Title
- Development and characterization of 37 novel EST-SSR markers in Pisum sativum (Fabaceae)
- Creators
- Xiaofeng Zhuang - Department of Plant, Soil and Microbial Sciences, Michigan State University, 107 CIPS, East Lansing, Michigan 48824 USAKevin E McPhee - Department of Plant Sciences, North Dakota State University, 370G Loftsgard Hall, Fargo, North Dakota 58108 USATristan E Coram - Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, Indiana 46268 USATobin L Peever - Department of Plant Pathology, Washington State University, Pullman, Washington 99164 USAMartin I Chilvers - Department of Plant, Soil and Microbial Sciences, Michigan State University, 107 CIPS, East Lansing, Michigan 48824 USA
- Publication Details
- Applications in plant sciences, Vol.1(1), pp.1200249-n/a
- Academic Unit
- Plant Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900547346301842
- Language
- English
- Resource Type
- Journal article