Journal article
Development and characterization of microsatellite markers of the fungal plant pathogen Sclerotinia trifoliorum
Genome, Vol.53(6), pp.494-500
06/2010
Handle:
https://hdl.handle.net/2376/101636
PMID: 20555438
Abstract
Sclerotinia trifoliorum
is an important pathogen of forage legumes and some grain legumes. Attempts to study its population biology using microsatellite markers developed for
Sclerotinia sclerotiorum
and
Sclerotinia subarctica
resulted in no amplification or low levels of polymorphism. This study reports the development and characterization of 33 microsatellite loci developed from a microsatellite-enriched library of S. trifoliorum. Based on a population of 42 isolates of S. trifoliorum, these microsatellite markers are highly polymorphic, with a mean of 6.5 alleles per locus (range 3-12) and a mean expected heterozygosity of 0.63 (range 0.26-0.9). Based on locations of these marker sequences in the S. sclerotiorum genome, these microsatellite loci are dispersed throughout the genome. However, 50% (265 of 528) of pairwise comparisons of the 33 microsatellite loci had significant linkage disequilibrium, which could be explained by the mixed mating systems (homothallism and heterothallism) and clonal reproduction of S. trifoliorum. Thirty of the 33 loci were successfully applied to S. sclerotiorum, and 28 loci were polymorphic. However, only 10 loci are applicable to
Sclerotinia minor
and 1 locus to
Sclerotinia homoeocarpa
. These markers are therefore useful for population structure assessment, QTL mapping, and ecological analyses in S. trifoliorum and potentially in other
Sclerotinia
species.
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Details
- Title
- Development and characterization of microsatellite markers of the fungal plant pathogen Sclerotinia trifoliorum
- Creators
- Evans Nyaga Njambere - Rutgers University, Department of Plant Biology and Pathology, 59 Dudley Road, Foran Hall 201, New Brunswick, NJ 08901, USAGeorge Vandemark - Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USAWeidong Chen - Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA
- Publication Details
- Genome, Vol.53(6), pp.494-500
- Academic Unit
- Plant Pathology, Department of
- Identifiers
- 99900546546901842
- Language
- English
- Resource Type
- Journal article