Journal article
Development and evaluation of a novel experimental system to control rosette leaf initiation in Arabidopsis
Plant science (Limerick), Vol.168(1), pp.37-44
2005
Handle:
https://hdl.handle.net/2376/116071
Abstract
A novel experimental system was developed to control rosette leaf initiation in
Arabidopsis. The system has simple constituents of a sequential culture of transgenic
Arabidopsis carrying a hygromycin resistance gene driven by the promoter of a nopaline synthase gene (
NOS::HPT) on a medium with or without 30
mg
l
−1 of hygromycin B, an antibiotic. Rosette leaf initiation of
NOS::HPT seedlings was severely arrested in the presence of hygromycin B. Leaves were re-initiated in more than 80% of the
NOS::HPT population when relieved from antibiotic influence. Expression profiles of leaf marker genes
AtEXP10,
AtmybL2, and
GL2 indicate that leaf re-initiation occurred in a synchronous fashion. Moreover, application of hygromycin B resulted in enlargement of the
NOS::HPT cotyledonous seedlings, which allowed us to dissect and collect a large amount of shoot apical tissue for molecular analysis. Taken together, our system provides a new opportunity to study functions of vegetative meristems in
Arabidopsis during leaf development with biochemical and molecular biological techniques in addition to genetic techniques.
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Details
- Title
- Development and evaluation of a novel experimental system to control rosette leaf initiation in Arabidopsis
- Creators
- Shigehisa Okamoto - Department of Agricultural Science, Kagoshima University, Kohrimoto 1-21-24, Kagoshima 890-0065, JapanKiwamu Tanaka - Department of Agricultural Science, Kagoshima University, Kohrimoto 1-21-24, Kagoshima 890-0065, JapanKatsuya Tsuruyama - Department of Biochemical Science and Technology, Kagoshima University, Kohrimoto 1-21-24, Kagoshima 890-0065, JapanYasushi Nakamura - Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, Shimogamo, Sakyo-ku, Kyoto 606-8522, JapanTomoaki Matsuo - Department of Biochemical Science and Technology, Kagoshima University, Kohrimoto 1-21-24, Kagoshima 890-0065, Japan
- Publication Details
- Plant science (Limerick), Vol.168(1), pp.37-44
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Elsevier Ireland Ltd
- Identifiers
- 99900547730101842
- Language
- English
- Resource Type
- Journal article