Journal article
Development and test of 21 multiplex PCRs composed of SSRs spanning most of the apple genome
Tree genetics & genomes, Vol.5(1), pp.211-223
2009
Handle:
https://hdl.handle.net/2376/116649
Abstract
A series of 21 multiplex (MP) polymerase chain reactions containing simple sequence repeat (SSR) markers spanning most of the apple genome has been developed. Eighty-eight SSR markers, well distributed over all 17 linkage groups (LGs), have been selected. Eighty-four of them were included in 21 different MPs while four could not be included in any MPs. The 21 MPs were then used to genotype approximately 2,000 DNA samples from the European High-quality Disease-Resistant Apples for a Sustainable agriculture project. Two SSRs (CH01d03 and NZAL08) were discarded at an early stage as they did not produce stable amplifications in the MPs, while the scoring of the multilocus (ML) SSR Hi07d11 and CN44794 was too complex for large-scale genotyping. The testing of the remaining 80 SSRs over a large number of different genotypes allowed: (1) a better estimation of their level of polymorphism; as well as of (2) the size range of the alleles amplified; (3) the identification of additional unmapped loci of some ML SSRs; (4) the development of methods to assign alleles to the different loci of ML SSRs and (5) conditions at which an SSR previously described as ML would amplify alleles of a single locus to be determined. These data resulted in the selection of 75 SSRs out of the 80 that are well suited and recommended for large genotyping projects
Metrics
9 Record Views
Details
- Title
- Development and test of 21 multiplex PCRs composed of SSRs spanning most of the apple genome
- Creators
- Andrea Patocchi - Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology in Zürich [Zürich]F Fernandez-Fernandez - East Malling ResearchK Evans - East Malling ResearchD Gobbin - Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology in Zürich [Zürich]F Rezzonico - Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology in Zürich [Zürich]A Boudichevskaia - Julius Kühn-InstitutF Dunemann - Julius Kühn-InstitutM Stankiewicz-Kosyl - Warsaw Agricultural UniversityFabienne Mathis - Unité mixte de recherche génétique et horticulture GenhortCharles Eric Durel - Unité mixte de recherche génétique et horticulture GenhortL Gianfranceschi - Department of Biomolecular Sciences and BiotechnologyFabrizio Costa - Alma Mater Studiorum Università di Bologna [Bologna]C Toller - Istituto Agrario di San Michele all'AdigeV Cova - Istituto Agrario di San Michele all'AdigeD Mott - Istituto Agrario di San Michele all'AdigeM Komjanc - Istituto Agrario di San Michele all'AdigeE Barbaro - Istituto Agrario di San Michele all'AdigeL Kodde - Wageningen University and Research CentreE Rikkerink - Horticulture and Food Research Institute of New ZealandCesare Gessler - Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology in Zürich [Zürich]W.E Van de Weg - Wageningen University and Research Centre
- Publication Details
- Tree genetics & genomes, Vol.5(1), pp.211-223
- Academic Unit
- WSU Wenatchee Tree Fruit REC
- Publisher
- Springer Verlag
- Identifiers
- 99900547521901842
- Language
- English
- Resource Type
- Journal article