Journal article
Development of Two Animal Models To Study the Function of Vibrio parahaemolyticus Type III Secretion Systems
Infection and immunity, Vol.78(11), pp.4551-4559
11/2010
Handle:
https://hdl.handle.net/2376/105536
PMCID: PMC2976342
PMID: 20823199
Abstract
Vibrio parahaemolyticus
is an emerging food- and waterborne pathogen that encodes two type III secretion systems (T3SSs). Previous studies have linked type III secretion system 1 (T3SS1) to cytotoxicity and T3SS2 to intestinal fluid accumulation, but animal challenge models needed to study these phenomena are limited. In this study we evaluated the roles of the T3SSs during infection using two novel animal models: a model in which piglets were inoculated orogastrically and a model in which mice were inoculated in their lungs (intrapulmonarily). The bacterial strains employed in this study had equivalent growth rates and beta-hemolytic activity based on
in vitro
assays. Inoculation of 48-h-old conventional piglets with 10
11
CFU of the wild-type strain (NY-4) or T3SS1 deletion mutant strains resulted in acute, self-limiting diarrhea, whereas inoculation with a T3SS2 deletion mutant strain failed to produce any clinical symptoms. Intrapulmonary inoculation of C57BL/6 mice with the wild-type strain and T3SS2 deletion mutant strains (5 × 10
5
CFU) induced mortality or a moribund state within 12 h (80 to 100% mortality), whereas inoculation with a T3SS1 deletion mutant or a T3SS1 T3SS2 double deletion mutant produced no mortality. Bacteria were recovered from multiple organs regardless of the strain used in the mouse model, indicating that the mice were capable of clearing the lung infection in the absence of a functional T3SS1. Because all strains had a similar beta-hemolysin phenotype, we surmise that thermostable direct hemolysin (TDH) plays a limited role in these models. The two models introduced herein produce robust results and provide a means to determine how different T3SS1 and T3SS2 effector proteins contribute to pathogenesis of
V. parahaemolyticus
infection.
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Details
- Title
- Development of Two Animal Models To Study the Function of Vibrio parahaemolyticus Type III Secretion Systems
- Creators
- Pablo Piñeyro - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Xiaohui Zhou - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Lisa H Orfe - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Patrick J Friel - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Kevin Lahmers - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Douglas R Call - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040
- Publication Details
- Infection and immunity, Vol.78(11), pp.4551-4559
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900546729601842
- Language
- English
- Resource Type
- Journal article