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Development of a multiplex real-time PCR for detection and differentiation of malignant catarrhal fever viruses in clinical samples
Journal article   Open access  Peer reviewed

Development of a multiplex real-time PCR for detection and differentiation of malignant catarrhal fever viruses in clinical samples

Cristina W Cunha, Lisa Otto, Naomi S Taus, Donald P Knowles and Hong Li
Journal of clinical microbiology, Vol.47(8), pp.2586-2589
08/2009
DOI: https://doi.org/10.1128/JCM.00997-09
Handle:
https://hdl.handle.net/2376/104233
PMCID: PMC2725674
PMID: 19494077
url
https://doi.org/10.1128/JCM.00997-09View
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Abstract

Herpesviridae - isolation & purification Humans Herpesviridae Infections - veterinary Molecular Sequence Data DNA Primers - genetics Herpesviridae Infections - diagnosis Polymerase Chain Reaction - methods Sequence Alignment Animals Lymphoproliferative Disorders - diagnosis Base Sequence Sensitivity and Specificity Herpesviridae - genetics Ruminants - virology Herpesviridae - classification Lymphoproliferative Disorders - veterinary
A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).

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