Development of a waterborne challenge model for Flavobacterium psychrophilum

Amy Long; Tyson R Fehringer; Benjamin R LaFrentz; Douglas R Call; Kenneth D Cain

doi:10.1111/1574-6968.12563

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Development of a waterborne challenge model for Flavobacterium psychrophilum

Journal article

Open access

Peer reviewed

Development of a waterborne challenge model for Flavobacterium psychrophilum

Amy Long, Tyson R Fehringer, Benjamin R LaFrentz, Douglas R Call and Kenneth D Cain

FEMS microbiology letters, Vol.359(2), pp.154-160

10/2014

DOI: https://doi.org/10.1111/1574-6968.12563

Handle:

https://hdl.handle.net/2376/101459

PMID: 25091473

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Abstract

Fish Diseases - microbiology

Fish Diseases - mortality

Virulence

Flavobacteriaceae Infections - mortality

Flavobacterium - pathogenicity

Flavobacteriaceae Infections - microbiology

Flavobacteriaceae Infections - veterinary

Iron - analysis

Animals

Oncorhynchus mykiss - microbiology

Models, Animal

Culture Media - chemistry

Aquaculture

Flavobacterium psychrophilum is the causative agent of bacterial coldwater disease and can cause significant mortality in salmonid aquaculture. To better evaluate disease prevention or treatment methods for F. psychrophilum in the laboratory, a waterborne challenge model that mimics a natural outbreak is needed. Here we report on the development of a waterborne challenge model for F. psychrophilum in which we incorporated variables that may influence challenge success: specifically, scarification prior to bacterial exposure and culture of F. psychrophilum under iron-limited culture conditions to potentially increase the probability of establishing disease. Additionally, two F. psychrophilum strains, CSF 259-93 and THC 02-90, were used in this model to test whether there were virulence differences between strains. Mortality was significantly higher in scarred fish than unscarred fish (81.5 vs. 19.4%), supporting the hypothesis that disruptions in the dermal layer enhance mortality in F. psychrophilum waterborne challenges. Although mortality differences were not significant between iron-replete and iron-limited treatments, mortality was high overall (> 30%). There was a significant difference in mortality between CSF 259-93 and THC 02-90 treatments, although both strains caused high mortality in injection challenges. In conclusion, this waterborne challenge model can be used to evaluate potential disease prevention and treatment methods.

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Title: Development of a waterborne challenge model for Flavobacterium psychrophilum
Creators: Amy Long - Department of Fish & Wildlife Sciences and the Aquaculture Research Institute, University of Idaho, Moscow, ID, USA
Tyson R Fehringer
Benjamin R LaFrentz
Douglas R Call
Kenneth D Cain
Publication Details: FEMS microbiology letters, Vol.359(2), pp.154-160
Academic Unit: Center for Reproductive Biology; Paul G. Allen School for Global Animal Health
Publisher: England
Identifiers: 99900546688101842
Language: English
Resource Type: Journal article