Journal article
Development of acetylcholinesterase biosensor based on CdTe quantum dots/gold nanoparticles modified chitosan microspheres interface
Biosensors & bioelectronics, Vol.24(3), pp.475-479
2008
Handle:
https://hdl.handle.net/2376/111541
PMID: 18640026
Abstract
In this paper, a novel acetylcholinesterase (AChE) biosensor was constructed by modifying glassy carbon electrode with CdTe quantum dots (QDs) and excellent conductive gold nanoparticles (GNPs) though chitosan microspheres to immobilize AChE. Since GNPs have shown widespread use particularly for constructing electrochemical biosensors through their high electron-transfer ability, the combined AChE exhibited high affinity to its substrate and thus a sensitive, fast and cheap method for determination of monocrotophos. The combination of CdTe QDs and GNPs promoted electron transfer and catalyzed the electro-oxidation of thiocholine, thus amplifying the detection sensitivity. This novel biosensing platform based on CdTe QDs–GNPs composite responded even more sensitively than that on CdTe QDs or GNPs alone because of the presence of synergistic effects in CdTe-GNPs film. The inhibition of monocrotophos was proportional to its concentration in two ranges, from 1 to 1000
ng
mL
−1 and from 2 to 15
μg
mL
−1, with a detection limit of 0.3
ng
mL
−1. The proposed biosensor showed good precision and reproducibility, acceptable stability and accuracy in garlic samples analysis.
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Details
- Title
- Development of acetylcholinesterase biosensor based on CdTe quantum dots/gold nanoparticles modified chitosan microspheres interface
- Creators
- Dan Du - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, ChinaShizhen Chen - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, ChinaDandan Song - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, ChinaHaibing Li - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, ChinaXi Chen - State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China
- Publication Details
- Biosensors & bioelectronics, Vol.24(3), pp.475-479
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900547716501842
- Language
- English
- Resource Type
- Journal article