Journal article
Developmental regulation of the gene for chimeric calcium/calmodulin-dependent protein kinase in anthers
Planta, Vol.209(2), pp.161-171
08/1999
Handle:
https://hdl.handle.net/2376/103110
PMID: 10436217
Abstract
Chimeric Ca2+/calmodulin-dependent protein kinase (CCaMK) was cloned from developing anthers of lily (Lilium longiflorum Thumb. cv. Nellie White) and tobacco (Nicotiana tabacum L. cv. Xanthi). Previous biochemical characterization and structure/function studies had revealed that CCaMK has dual modes of regulation by Ca2+ and Ca2+/calmodulin. The unique structural features of CCaMK include a catalytic domain, a calmodulin-binding domain, and a neural visinin-like Ca2+-binding domain. The existence of these three features in a single polypeptide distinguishes it from other kinases. Western analysis revealed that CCaMK is expressed in a stage-specific manner in developing anthers. Expression of CCaMK was first detected in pollen mother cells and continued to increase, reaching a peak around the tetrad stage of meiosis. Following microsporogenesis, CCaMK expression rapidly decreased and at later stages of microspore development, no expression was detected. A tobacco genomic clone of CCaMK was isolated and transgenic tobacco plants were produced carrying the CCaMK promoter fused to the β-glucuronidase reporter gene. Both CCaMK mRNA and protein were detected in the pollen sac and their localizations were restricted to the pollen mother cells and tapetal cells. Consistent results showing a stage-specific expression pattern were obtained by β-glucuronidase analysis, in-situ hybridization and immunolocalization. The stage- and tissue-specific appearance of CCaMK in anthers suggests that it could play a role in sensing transient changes in free Ca2+ concentration in target cells, thereby controlling developmental events in the anther.
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Details
- Title
- Developmental regulation of the gene for chimeric calcium/calmodulin-dependent protein kinase in anthers
- Creators
- B. W Poovaiah - Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture, Washington State University, Pullman, WA 99164, USA USMian Xia - Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture, Washington State University, Pullman, WA 99164, USA USZhihua Liu - Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture, Washington State University, Pullman, WA 99164, USA USWuyi Wang - Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture, Washington State University, Pullman, WA 99164, USA USTianbao Yang - Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture, Washington State University, Pullman, WA 99164, USA USP. V Sathyanarayanan - Laboratory of Plant Molecular Biology and Physiology, Department of Horticulture, Washington State University, Pullman, WA 99164, USA USVincent R Franceschi - Department of Botany and Electron Microscopy Center, Washington State University, Pullman, WA 99164, USA US
- Publication Details
- Planta, Vol.209(2), pp.161-171
- Academic Unit
- Department of Horticulture
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900546503401842
- Language
- English
- Resource Type
- Journal article