Journal article
Developmental regulation of translation by the 5' noncoding region of murine c-myc mRNA in Xenopus laevis
Oncogene, Vol.3(5), pp.517-521
11/1988
Handle:
https://hdl.handle.net/2376/106367
PMID: 3078957
Abstract
The translational efficiency of chloramphenicol acetyltransferase (CAT) mRNA containing a 5' noncoding sequence derived from exon 1 of the murine c-myc gene (360CAT) has been examined at different stages of Xenopus egg development. In contrast to its reduced translation in the Xenopus oocyte, 360CAT mRNA is translated as efficiently as CAT mRNA when injected into either mature Xenopus eggs or Xenopus embryos. No significant alteration of 360CAT mRNA stability was observed up to 10 h post-fertilization in Xenopus embryos as compared to that of CAT mRNA. The increase in 360CAT mRNA translational efficiency in Xenopus embryos was not observed with CAT mRNAs possessing other inhibitory 5' noncoding sequences. The increase in 360CAT mRNA translational efficiency is attributed to a trans-acting factor synthesized or activated following Xenopus oocyte maturation. The possible significance of the 5' noncoding region of c-myc mRNA in developmental expression of c-myc is discussed.
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Details
- Title
- Developmental regulation of translation by the 5' noncoding region of murine c-myc mRNA in Xenopus laevis
- Creators
- P Lazarus - Department of Biochemistry, McGill University, Montreal, CanadaN ParkinN Sonenberg
- Publication Details
- Oncogene, Vol.3(5), pp.517-521
- Publisher
- England
- Identifiers
- 99900546703001842
- Language
- English
- Resource Type
- Journal article