Journal article
Developmental stage-specific metabolic and transcriptional activity of Chlamydia trachomatis in an axenic medium
Proceedings of the National Academy of Sciences - PNAS, Vol.109(48), pp.19781-19785
From the Cover
11/27/2012
Handle:
https://hdl.handle.net/2376/112851
PMCID: PMC3511728
PMID: 23129646
Abstract
Chlamydia trachomatis
is among the most clinically significant human pathogens, yet their obligate intracellular nature places severe restrictions upon research. Chlamydiae undergo a biphasic developmental cycle characterized by an infectious cell type known as an elementary body (EB) and an intracellular replicative form called a reticulate body (RB). EBs have historically been described as metabolically dormant. A cell-free (axenic) culture system was developed, which showed high levels of metabolic and biosynthetic activity from both EBs and RBs, although the requirements differed for each. EBs preferentially used glucose-6-phosphate as an energy source, whereas RBs required ATP. Both developmental forms showed increased activity when incubated under microaerobic conditions. Incorporation of isotopically labeled amino acids into proteins from both developmental forms indicated unique expression profiles, which were confirmed by genome-wide transcriptional analysis. The described axenic culture system will greatly enhance biochemical and physiological analyses of chlamydiae.
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Details
- Title
- Developmental stage-specific metabolic and transcriptional activity of Chlamydia trachomatis in an axenic medium
- Creators
- Anders Omsland - Host–Parasite Interactions Section, Laboratory of Intracellular ParasitesJanet Sager - Host–Parasite Interactions Section, Laboratory of Intracellular ParasitesVinod Nair - Electron Microscopy Unit, andDaniel E Sturdevant - Genomics Unit, Research Technologies Section, Rocky Mountain LaboratoriesTed Hackstadt - Host–Parasite Interactions Section, Laboratory of Intracellular Parasites
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.109(48), pp.19781-19785
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Series
- From the Cover
- Publisher
- National Academy of Sciences
- Identifiers
- 99900547449001842
- Language
- English
- Resource Type
- Journal article