Journal article
Dictyostelium cell shape generation requires myosin II
Cell motility and the cytoskeleton, Vol.35(1), pp.59-67
1996
Handle:
https://hdl.handle.net/2376/106369
PMID: 8874966
Abstract
Myosin II function has been implicated in non‐muscle cell behaviors including movement, cytokinesis, and multicellular morphogenesis. Surprisingly, two‐dimensional morphology and behavior of Dictyostelium amoebae lacking myosin II (mhcA−) is not dramatically altered when cells are examined using conventional imaging techniques. We have observed amoebae from the side (side‐view microscopy or SVM) and find that wild‐type but not mhcA− cells undergo extensive three‐dimensional (3D) shape changes. These shape changes often occur above the plane of the substrate and are morphologically distinct from pseudopods. For example, unlike pseudopods, vertically extended cell regions are not enriched in F‐actin and do not exclude organelles. In contrast, mhcA− cells generate F‐actin‐filled pseudopods and spread laterally but do not undergo vertical extension. When wild‐type cells are removed from the substrate and shaken in suspension, they retain their irregular three‐dimensional shape. Suspended mhcA− cells, however, rapidly become spherical. Thus, cells lacking myosin appear to be incapable of generating or maintaining 3D shape independent of a substrate. When a substrate is available, these cells can attach and spread on the surface in a myosin‐independent manner. These previously undescribed defects of mhcA− cells reveal that 3D cell shape generation requires myosin II and is mechanistically distinct from pseudopod formation. © l996 Wiley‐Liss, Inc.
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Details
- Title
- Dictyostelium cell shape generation requires myosin II
- Creators
- Eric SheldenDavid A Knecht
- Publication Details
- Cell motility and the cytoskeleton, Vol.35(1), pp.59-67
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- Number of pages
- 9
- Identifiers
- 99900546711401842
- Language
- English
- Resource Type
- Journal article