Journal article
Dielectrophoretic characterization of antibiotic-treated Mycobacterium tuberculosis complex cells
Analytical and bioanalytical chemistry, Vol.407(25), pp.7673-7680
10/2015
Handle:
https://hdl.handle.net/2376/101951
PMID: 26231690
Abstract
Multi-drug resistant tuberculosis (MDR-TB) has become a serious concern for proper treatment of patients. As a phenotypic method, dielectrophoresis can be useful but is yet to be attempted to evaluate Mycobacterium tuberculosis complex cells. This paper investigates the dielectrophoretic behavior of Mycobacterium bovis (Bacillus Calmette-Guérin, BCG) cells that are treated with heat or antibiotics rifampin (RIF) or isoniazid (INH). The experimental parameters are designed on the basis of our sensitivity analysis. The medium conductivity (σ(m)) and the frequency (f) for a crossover frequency (f(xo1)) test are decided to detect the change of σ(m)-f(xo1) in conjunction with the drug mechanism. Statistical modeling is conducted to estimate the distributions of viable and nonviable cells from the discrete measurement of f (xo1). Finally, the parameters of the electrophysiology of BCG cells, C(envelope) and σ(cyto), are extracted through a sampling algorithm. This is the first evaluation of the dielectrophoresis (DEP) approach as a means to assess the effects of antimicrobial drugs on M. tuberculosis complex cells.
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Details
- Title
- Dielectrophoretic characterization of antibiotic-treated Mycobacterium tuberculosis complex cells
- Creators
- Shinnosuke Inoue - Department of Mechanical Engineering, University of Washington, Stevens Way, Box 352600, Seattle, WA, 98195, USAHyun-Boo Lee - Department of Mechanical Engineering, University of Washington, Stevens Way, Box 352600, Seattle, WA, 98195, USAAnnie L Becker - Department of Environmental and Occupational Health Sciences, University of Washington, 1959 Pacific Street, Box 357234, Seattle, WA, 98195, USAKris M Weigel - Department of Environmental and Occupational Health Sciences, University of Washington, 1959 Pacific Street, Box 357234, Seattle, WA, 98195, USAJong-Hoon Kim - School of Engineering and Computer Science, Washington State University, 14204 NE Salmon Creek Avenue, Vancouver, WA, 98686, USAKyong-Hoon Lee - NanoFacture, Inc., P.O. Box 52651, Bellevue, WA, 98015, USAGerard A Cangelosi - Department of Environmental and Occupational Health Sciences, University of Washington, 1959 Pacific Street, Box 357234, Seattle, WA, 98195, USAJae-Hyun Chung - Department of Mechanical Engineering, University of Washington, Stevens Way, Box 352600, Seattle, WA, 98195, USA. jae71@uw.edu
- Publication Details
- Analytical and bioanalytical chemistry, Vol.407(25), pp.7673-7680
- Publisher
- Germany
- Identifiers
- 99900546618101842
- Language
- English
- Resource Type
- Journal article