Journal article
Dielectrophoretic concentration of low-abundance nanoparticles using a nanostructured tip
Nanotechnology, Vol.23(48), pp.485707-485707
11/09/2012
Handle:
https://hdl.handle.net/2376/104493
PMID: 23137928
Abstract
Electric field-induced concentration has the potential for application in highly sensitive detection of nanoparticles (NPs) for disease diagnosis and drug discovery. Conventional two-dimensional planar electrodes, however, have shown limited sensitivity in NP concentration. In this paper, the dielectrophoretic (DEP) concentration of low-abundance NPs is studied using a nanostructured tip where a high electric field of 3 × 107 V m−1 is generated. In experimental studies, individual 2, 10, and 100 nm Au NPs are concentrated to a nanotip using DEP concentration and are detected by scanning transmission and scanning electron microscopes. The DEP force on Au NPs near the end of a nanotip is computed according to the distance, and then compared with Brownian motion-induced force. The computational study shows qualitative agreement with the experimental results. When the experimental conditions for DEP concentration are optimized for 8 nm-long oligonucleotides, the sensitivity of a nanotip is 10 aM (10 attomolar; nine copies in a 1.5 μl sample volume). This DEP concentrator using a nanotip can be used for molecular detection without amplification.
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Details
- Title
- Dielectrophoretic concentration of low-abundance nanoparticles using a nanostructured tip
- Creators
- Woon-Hong Yeo - University of Washington Department of Mechanical Engineering, Seattle, WA 98195, USAAdrian M Kopacz - Northwestern University Department of Mechanical Engineering, Evanston, IL 60208, USAJong-Hoon Kim - University of Washington Department of Mechanical Engineering, Seattle, WA 98195, USAXinqi Chen - Northwestern UniversityJinsong Wu - Northwestern University NUANCE Center, Evanston, IL 60208, USADayong Gao - University of Washington Department of Mechanical Engineering, Seattle, WA 98195, USAKyong-Hoon Lee - NanoFacture, Inc. , PO Box 52650, Bellevue, WA 98015, USAWing-Kam Liu - World Class University (WCU) Programme in Sungkyunkwan University School of Mechanical Engineering, KoreaJae-Hyun Chung - University of Washington Department of Mechanical Engineering, Seattle, WA 98195, USA
- Publication Details
- Nanotechnology, Vol.23(48), pp.485707-485707
- Academic Unit
- School of Engineering and Computer Science (VANC)
- Publisher
- IOP Publishing
- Number of pages
- 7
- Identifiers
- 99900546843601842
- Language
- English
- Resource Type
- Journal article