Journal article
Dietary Supplementation of Vitamin E and α-Lipoic Acid Upregulates Cell Growth and Signaling Genes in Rat Myocardium
International journal of biomedical science, Vol.2(4), pp.356-359
12/2006
Handle:
https://hdl.handle.net/2376/107797
PMCID: PMC3614636
PMID: 23675004
Abstract
The efficacy of antioxidant supplementation in the prevention of cardiovascular disease appears equivocal, however the use of more potent antioxidant combinations than those traditionally used may exert a more positive effect. We have shown previously that supplementation of vitamin E and α-lipoic acid increases cardiac performance during post-ischemia reperfusion in older rats and increases Bcl-2 levels in endothelial cells. The purpose of this study was to examine the effects of vitamin E and α-lipoic acid supplementation on myocardial gene expression with a view to determine their mechanism of action. Young male rats received either a control (n=7) or vitamin E and α-lipoic acid supplemented diet (n=8) for 14 weeks. RNA from myocardial tissue was then amplified and samples were pooled within groups and competitively hybridized to 8.5K oligonucleotide rat microarrays. The relative expression of each gene was then compared to the control sample. Animals that received the antioxidant-supplemented diet exhibited upregulation (>1.5×) of 13 genes in the myocardium with 2 genes downregulated. Upregulated genes include those involved in cell growth and maintenance (LynB, Csf1r, Akt2, Tp53), cell signaling (LynB, Csf1r) and signal transduction (Pacsin2, Csf1r). Downregulated genes encode thyroid (Thrsp) and F-actin binding proteins (Nexilin).
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Details
- Title
- Dietary Supplementation of Vitamin E and α-Lipoic Acid Upregulates Cell Growth and Signaling Genes in Rat Myocardium
- Creators
- Susan A Marsh - School of Human Movement Studies, The University of Queensland, Brisbane 4072, AustraliaSteven MasonLeigh C WardJeff S Coombes
- Publication Details
- International journal of biomedical science, Vol.2(4), pp.356-359
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- United States
- Identifiers
- 99900546743001842
- Language
- English
- Resource Type
- Journal article