Journal article
Dietary carbohydrate level affects transcription factor expression that regulates skeletal muscle myogenesis in rainbow trout
Comparative Biochemistry and Physiology, Part B, Vol.153(1), pp.66-72
2009
Handle:
https://hdl.handle.net/2376/108374
PMID: 19416696
Abstract
Understanding the effects of dietary carbohydrates on transcription factors that regulate myogenesis provides insight into the role of nutrient sensing by satellite cells towards myocyte differentiation. We evaluated the influence of dietary carbohydrate level (0, 15, 25 or 35%) on the temporal mRNA expression patterns (4, 8 or 12 weeks) of transcription factors that regulate satellite cell myocyte addition (MA) in rainbow trout (
Oncorhynchus mykiss), a vertebrate with indeterminate growth. Relative to the 0% carbohydrate (NC) diet, 15 (IC-15) and 25% (IC-25) carbohydrate containing diets significantly up-regulate
MyoD and
Myf5, but not
Pax7, after 12 weeks of feeding. Simultaneously, the
Pax7/
MyoD mRNA expression ratio declined significantly with both the IC diets.
Myogenin mRNA expression also increased in rainbow trout (RBT) fed the IC-15 diet. The high carbohydrate (HC) diet (35%) attenuated the increased mRNA expression of these transcription factors. It is of note that the 4 and 8 week samples lacked the promyogenic expression patterns. The myogenic gene expression in fish fed the IC-15 diet for 12 weeks indicate a transcriptional signature that reflects increased satellite cell myogenesis. Our results suggest a potential role for satellite cells in the nutrient sensing ability of a vertebrate with indeterminate skeletal muscle growth.
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Details
- Title
- Dietary carbohydrate level affects transcription factor expression that regulates skeletal muscle myogenesis in rainbow trout
- Creators
- Kalyan C Chapalamadugu - Department of Animal and Veterinary Science, University of Idaho, Moscow, Idaho 83844, USABarrie D Robison - Department of Biological Sciences, University of Idaho, Moscow, Idaho 83844, USARobert E Drew - Department of Biological Sciences, University of Idaho, Moscow, Idaho 83844, USAMadison S Powell - Aquaculture Research Institute, University of Idaho, Hagerman, Idaho 83332, USARodney A Hill - Department of Animal and Veterinary Science, University of Idaho, Moscow, Idaho 83844, USAJon J Amberg - Department of Animal and Veterinary Science, University of Idaho, Moscow, Idaho 83844, USAKenneth J Rodnick - Department of Biological Sciences, Idaho State University, Pocatello, Idaho 83209, USARonald W Hardy - Aquaculture Research Institute, University of Idaho, Hagerman, Idaho 83332, USAMadison L Hill - Department of Animal and Veterinary Science, University of Idaho, Moscow, Idaho 83844, USAGordon K Murdoch - Department of Animal and Veterinary Science, University of Idaho, Moscow, Idaho 83844, USA
- Publication Details
- Comparative Biochemistry and Physiology, Part B, Vol.153(1), pp.66-72
- Academic Unit
- Animal Sciences, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900546911001842
- Language
- English
- Resource Type
- Journal article