Journal article
Differential Expression and Sequence Conservation of the Anaplasma marginale msp2 Gene Superfamily Outer Membrane Proteins
Infection and immunity, Vol.74(6), pp.3471-3479
06/2006
Handle:
https://hdl.handle.net/2376/111728
PMCID: PMC1479288
PMID: 16714578
Abstract
Bacterial pathogens in the genera
Anaplasma
and
Ehrlichia
encode a protein superfamily, pfam01617, which includes the predominant outer membrane proteins (OMPs) of each species, major surface protein 2 (MSP2) and MSP3 of
Anaplasma marginale
and
Anaplasma ovis
,
Anaplasma phagocytophilum
MSP2 (p44),
Ehrlichia chaffeensis
p28-OMP,
Ehrlichia canis
p30, and
Ehrlichia ruminantium
MAP1, and has been shown to be involved in both antigenic variation within the mammalian host and differential expression between the mammalian and arthropod hosts. Recently, complete sequencing of the
A. marginale
genome has identified an expanded set of genes, designated
omp1-14
, encoding new members of this superfamily. Transcriptional analysis indicated that, with the exception of the three smallest open reading frames,
omp2
,
omp3
, and
omp6
, these superfamily genes are transcribed in
A. marginale
-infected erythrocytes, tick midgut and salivary glands, and the IDE8 tick cell line. OMPs 1, 4, 7 to 9, and 11 were confirmed to be expressed as proteins by
A. marginale
within infected erythrocytes, with expression being either markedly lower (OMPs 1, 4, and 7 to 9) or absent (OMP11) in infected tick cells, which reflected regulation at the transcript level. Although the pfam01617 superfamily includes the antigenically variable MSP2 and MSP3 surface proteins, analysis of the
omp1-14
sequences throughout a cycle of acute and persistent infection in the mammalian host and tick transmission reveals a high degree of conservation, an observation supported by sequence comparisons between the St. Maries strain and Florida strain genomes.
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Details
- Title
- Differential Expression and Sequence Conservation of the Anaplasma marginale msp2 Gene Superfamily Outer Membrane Proteins
- Creators
- Susan M Noh - Program in Vector-Borne Diseases, Department of Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Kelly A Brayton - Program in Vector-Borne Diseases, Department of Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Donald P Knowles - Program in Vector-Borne Diseases, Department of Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Joseph T Agnes - Program in Vector-Borne Diseases, Department of Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Michael J Dark - Program in Vector-Borne Diseases, Department of Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Wendy C Brown - Program in Vector-Borne Diseases, Department of Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Timothy V Baszler - Program in Vector-Borne Diseases, Department of Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Guy H Palmer - Program in Vector-Borne Diseases, Department of Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040
- Publication Details
- Infection and immunity, Vol.74(6), pp.3471-3479
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547531501842
- Language
- English
- Resource Type
- Journal article