Journal article
Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition
International journal of biological sciences, Vol.5(1), pp.28-33
01/01/2009
Handle:
https://hdl.handle.net/2376/105979
PMCID: PMC2605576
PMID: 19119313
Abstract
Intramuscular fat (IMF) content plays a key role in establishing pork quality. In the present study, differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to identify differentially expressed (DE) genes between
longissimus dorsi
(LD) muscles with extremely different IMF content. A major DE gene associated with IMF content was identified as splicing factor serine-arginine rich protein (
SFRS18
) gene, also known as SRrp130. The gene exhibited relatively higher expression levels in LD muscles with higher IMF content. A full-length cDNA sequence of pig
SFRS18
gene was obtained by
in silico
comparative cloning coupled with PCR target sequencing, while the current EST (expressed sequence tag) database supported two transcript variants of the pig gene. Differential expression of the
SFRS18
gene was further confirmed using quantitative PCR. The mRNA levels of
SFRS18
gene showed significant and positive correlation with IMF content in LD muscle (r = 0.54, P < 0.01). Collectively, these results suggest that the
SFRS18
gene is involved in the regulation of IMF deposition in pig and that it may be a useful tool in selecting animals for desired amounts of fatness for high quality pork.
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Details
- Title
- Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition
- Creators
- Xiuxing Wang - 1. College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, ChinaChunyang Xue - 1. College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, ChinaXiaona Wang - 1. College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, ChinaHonglin Liu - 1. College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, ChinaYinxue Xu - 1. College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, ChinaRuqian Zhao - 2. Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing 210095, ChinaZhihua Jiang - 3. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAMichael V Dodson - 3. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAJie Chen - 1. College of Animal Science & Technology, Nanjing Agricultural University, Nanjing 210095, China
- Publication Details
- International journal of biological sciences, Vol.5(1), pp.28-33
- Academic Unit
- Animal Sciences, Department of
- Publisher
- Ivyspring International Publisher; Sydney
- Identifiers
- 99900546742601842
- Language
- English
- Resource Type
- Journal article