Differential effects of type I IFNs on the growth of WC1- CD8+ gamma delta T cells and WC1+ CD8- gamma delta T cells in vitro

W Tuo; F W Bazer; W C Davis; D Zhu; W C Brown

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Differential effects of type I IFNs on the growth of WC1- CD8+ gamma delta T cells and WC1+ CD8- gamma delta T cells in vitro

Journal article

Peer reviewed

Differential effects of type I IFNs on the growth of WC1- CD8+ gamma delta T cells and WC1+ CD8- gamma delta T cells in vitro

W Tuo, F W Bazer, W C Davis, D Zhu and W C Brown

The Journal of immunology (1950), Vol.162(1), pp.245-253

01/01/1999

Handle:

https://hdl.handle.net/2376/109330

PMID: 9886392

Abstract

CD8-Positive T-Lymphocytes - cytology

Interferon-alpha - pharmacology

Receptors, Antigen, T-Cell, gamma-delta - blood

T-Lymphocyte Subsets - cytology

Antigens, CD - biosynthesis

Humans

RNA, Messenger - biosynthesis

Interleukin-2 - physiology

Flow Cytometry

Cattle

Cell Division - immunology

Interferon Type I - pharmacology

T-Lymphocyte Subsets - drug effects

CD8-Positive T-Lymphocytes - metabolism

Female

Epitopes, T-Lymphocyte - immunology

Growth Inhibitors - pharmacology

Cell Line

Receptors, Antigen, T-Cell, gamma-delta - biosynthesis

CD8 Antigens - biosynthesis

Immunophenotyping

Down-Regulation - drug effects

Cell Division - drug effects

Up-Regulation - drug effects

Interleukin-2 - genetics

Animals

Up-Regulation - immunology

Down-Regulation - immunology

T-Lymphocyte Subsets - metabolism

CD8-Positive T-Lymphocytes - drug effects

Growth Substances - pharmacology

Sheep

Antigens, Differentiation, T-Lymphocyte - biosynthesis

Interleukin-2 - biosynthesis

Interferon-gamma - pharmacology

Type I IFNs have a broad array of immunoregulatory functions that include up-regulation of type 1 immune responses through enhancing differentiation and activation of CD8+ T cells and CD4+ Th1 cells. Ovine trophoblast IFN-tau is a recently described type I IFN with the potential for therapeutic use, based on its potent antiviral activity yet low toxicity. Studies were designed to determine the immunoregulatory effects of IFN-tau on Ag-stimulated T cells, and a novel effect of type I IFNs on gammadelta T cells was observed. In cultures of parasite Ag-stimulated bovine T cells that contained a mixture of alphabeta and gammadelta T cells, both IFN-tau and IFN-alpha suppressed the expansion of WC1+ CD2- CD6- CD8- gammadelta T cells, yet stimulated the growth of WC1- CD2+ CD6+ CD8+ gammadelta T cells and CD8+ alphabeta T cells. The CD8+ gammadelta T cell subset expressed high levels of the IL-2R alpha-chain. Furthermore, we showed that type I IFN enhanced IL-2 production by these Ag-stimulated T cell lines. In short term cultures of PBMC, IL-2 stimulated an expansion of WC1- CD6+ CD8+ gammadelta T cells, which was significantly increased by IFN-tau, even though IFN-tau alone did not support cell survival. These studies demonstrate for the first time that type I IFNs differentially modulate the proliferation of different subsets of gammadelta T cells, which appears to act in part via IL-2.

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Title: Differential effects of type I IFNs on the growth of WC1- CD8+ gamma delta T cells and WC1+ CD8- gamma delta T cells in vitro
Creators: W Tuo - Department of Veterinary Pathology and Microbiology, Washington State University, Pullman 99164, USA
F W Bazer
W C Davis
D Zhu
W C Brown
Publication Details: The Journal of immunology (1950), Vol.162(1), pp.245-253
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: United States
Grant note: R01AI30136 / NIAID NIH HHS
Identifiers: 99900547032101842
Language: English
Resource Type: Journal article

Differential effects of type I IFNs on the growth of WC1- CD8+ gamma delta T cells and WC1+ CD8- gamma delta T cells in vitro

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