Journal article
Differential expression of FAK and Pyk2 in metastatic and non-metastatic EL4 lymphoma cell lines
Clinical & experimental metastasis, Vol.28(6), pp.551-565
08/2011
Handle:
https://hdl.handle.net/2376/104990
PMCID: PMC3193847
PMID: 21533871
Abstract
The murine EL4 lymphoma cell line exists in variants that are either sensitive or resistant to phorbol 12-myristate 13-acetate (PMA). In sensitive cells, PMA causes Erk MAPK activation and Erk-mediated growth arrest. In resistant cells, PMA induces a low level of Erk activation, without growth arrest. A relatively unexplored aspect of the phenotypes is that resistant cells are more adherent to culture substrate than are sensitive cells. In this study, the roles of the protein tyrosine kinases FAK and Pyk2 in EL4 phenotype were examined, with a particular emphasis on the role of these proteins in metastasis. FAK is expressed only in PMA-resistant (or intermediate phenotype) EL4 cells, correlating with enhanced cell-substrate adherence, while Pyk2 is more highly expressed in non-adherent PMA-sensitive cells. PMA treatment causes modulation of mRNA for FAK (up-regulation) and Pyk2 (down-regulation) in PMA-sensitive but not PMA-resistant EL4 cells. The increase in Pyk2 mRNA is correlated with an increase in Pyk2 protein expression. The roles of FAK in cell phenotype were further explored using transfection and knockdown experiments. The results showed that FAK does not play a major role in modulating PMA-induced Erk activation in EL4 cells. However, the knockdown studies demonstrated that FAK expression is required for proliferation and migration of PMA-resistant cells. In an experimental metastasis model using syngeneic mice, only FAK-expressing (PMA-resistant) EL4 cells form liver tumors. Taken together, these studies suggest that FAK expression promotes metastasis of EL4 lymphoma cells.
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Details
- Title
- Differential expression of FAK and Pyk2 in metastatic and non-metastatic EL4 lymphoma cell lines
- Creators
- Zhihong Zhang - Program in Nutrition and Exercise Physiology, Washington State University, Spokane, WA, 99210-1495, USAStewart M KnoeppHsun KuHeather M SansburyYuhuan XieManpreet S ChahalStephen TomlinsonKathryn E Meier
- Publication Details
- Clinical & experimental metastasis, Vol.28(6), pp.551-565
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- Netherlands
- Grant note
- R01 CA094144-05 / NCI NIH HHS R01 CA094144 / NCI NIH HHS CA94144 / NCI NIH HHS CA58640 / NCI NIH HHS
- Identifiers
- 99900546725001842
- Language
- English
- Resource Type
- Journal article