Differential expression of three members of the multidomain adhesion CCp family in Babesia bigemina, Babesia bovis and Theileria equi

Reginaldo G Bastos; Carlos E Suarez; Jacob M Laughery; Wendell C Johnson; Massaro W Ueti; Donald P Knowles

doi:10.1371/journal.pone.0067765

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Differential expression of three members of the multidomain adhesion CCp family in Babesia bigemina, Babesia bovis and Theileria equi

Journal article

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Differential expression of three members of the multidomain adhesion CCp family in Babesia bigemina, Babesia bovis and Theileria equi

Reginaldo G Bastos, Carlos E Suarez, Jacob M Laughery, Wendell C Johnson, Massaro W Ueti and Donald P Knowles

PloS one, Vol.8(7), pp.e67765-e67765

2013

DOI: https://doi.org/10.1371/journal.pone.0067765

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https://hdl.handle.net/2376/107983

PMCID: PMC3701008

PMID: 23844089

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Abstract

Babesia - genetics

Gene Expression

Multigene Family

Gene Expression Regulation

Babesia - metabolism

Protozoan Proteins - genetics

Babesia bovis - metabolism

Theileria - metabolism

Protozoan Proteins - chemistry

Protein Interaction Domains and Motifs

Theileria - genetics

Babesia bovis - genetics

DNA, Complementary

Members of the CCp protein family have been previously described to be expressed on gametocytes of apicomplexan Plasmodium parasites. Knocking out Plasmodium CCp genes blocks the development of the parasite in the mosquito vector, making the CCp proteins potential targets for the development of a transmission-blocking vaccine. Apicomplexans Babesia bovis and Babesia bigemina are the causative agents of bovine babesiosis, and apicomplexan Theileria equi causes equine piroplasmosis. Bovine babesiosis and equine piroplasmosis are the most economically important parasite diseases that affect worldwide cattle and equine industries, respectively. The recent sequencing of the B. bovis and T. equi genomes has provided the opportunity to identify novel genes involved in parasite biology. Here we characterize three members of the CCp family, named CCp1, CCp2 and CCp3, in B. bigemina, B. bovis and T. equi. Using B. bigemina as an in vitro model, expression of all three CCp genes and proteins was demonstrated in temperature-induced sexual stages. Transcripts for all three CCp genes were found in vivo in blood stages of T. equi, and transcripts for CCp3 were detected in vivo in blood stages of B. bovis. However, no protein expression was detected in T. equi blood stages or B. bovis blood stages or B. bovis tick stages. Collectively, the data demonstrated a differential pattern of expression of three orthologous genes of the multidomain adhesion CCp family by B. bigemina, B. bovis and T. equi. The novel CCp members represent potential targets for innovative approaches to control bovine babesiosis and equine piroplasmosis.

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Title: Differential expression of three members of the multidomain adhesion CCp family in Babesia bigemina, Babesia bovis and Theileria equi
Creators: Reginaldo G Bastos - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America. rgbastos@vetmed.wsu.edu
Carlos E Suarez
Jacob M Laughery
Wendell C Johnson
Massaro W Ueti
Donald P Knowles
Publication Details: PloS one, Vol.8(7), pp.e67765-e67765
Academic Unit: Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: United States
Identifiers: 99900547076701842
Language: English
Resource Type: Journal article