Journal article
Differential mRNA stability to endogenous ribonucleases of the coding region and 3' untranslated regions of wheat (Triticum aestivum L.) manganese superoxide dismutase genes
Plant cell reports, Vol.25(2), pp.133-139
03/2006
Handle:
https://hdl.handle.net/2376/108876
PMID: 16240120
Abstract
The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenous nucleases. The degradation rates of the 3' UTR variants for 15 min were not significantly different, meaning the degradation rates of the 3' UTR variants were not directly related to the thermostabilities. However, the degradation rate of the coding region was significantly faster than those of the 3' UTR variants. Further investigation revealed the coding region seemed to have specific sites for degradation, indicating a possibility of increasing MnSOD expression by the degradation site alteration.
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Details
- Title
- Differential mRNA stability to endogenous ribonucleases of the coding region and 3' untranslated regions of wheat (Triticum aestivum L.) manganese superoxide dismutase genes
- Creators
- Kwang-Hyun Baek - Department of Crop and Soil Sciences, Washington State University, WA 99164-6420, USADaniel Z Skinner
- Publication Details
- Plant cell reports, Vol.25(2), pp.133-139
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- Germany
- Identifiers
- 99900547233001842
- Language
- English
- Resource Type
- Journal article