Journal article
Direct Comparison of Demyelinating Disease Induced by the Daniel’s Strain and BeAn Strain of Theiler’s Murine Encephalomyelitis Virus
Brain pathology (Zurich, Switzerland), Vol.13(3), pp.291-308
07/2003
Handle:
https://hdl.handle.net/2376/113828
PMCID: PMC5451092
PMID: 12946019
Abstract
We compared CNS disease following intracerebral injection of SJL mice with Daniel’s (DA) and BeAn 8386 (BeAn) strains of Theiler’s murine encephalomyelitis virus (TMEV). In tissue culture, DA was more virulent then BeAn. There was a higher incidence of demyelination in the spinal cords of SJL/J mice infected with DA as compared to BeAn. However, the extent of demyelination was similar between virus strains when comparing those mice that developed demyelination. Even though BeAn infection resulted in lower incidence of demyelination in the spinal cord, these mice showed significant brain disease similar to that observed with DA. There was approximately 100 times more virus specific RNA in the CNS of DA infected mice as compared to BeAn infected mice. This was reflected by more virus antigen positive cells (macrophages/microglia and oligodendrocytes) in the spinal cord white matter of DA infected mice as compared to BeAn. There was no difference in the brain infiltrating immune cells of DA or BeAn infected mice. However, BeAn infected mice showed higher titers of TMEV specific antibody. Functional deficits as measured by Rotarod were more severe in DA infected versus BeAn infected mice. These findings indicate that the diseases induced by DA or BeAn are distinct.
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Details
- Title
- Direct Comparison of Demyelinating Disease Induced by the Daniel’s Strain and BeAn Strain of Theiler’s Murine Encephalomyelitis Virus
- Creators
- Laurie J Zoecklein - Department of Immunology, Mayo Medical and Graduate Schools, Rochester, MinnKevin D Pavelko - Department of Immunology, Mayo Medical and Graduate Schools, Rochester, MinnJeff Gamez - Department of Immunology, Mayo Medical and Graduate Schools, Rochester, MinnLouisa Papke - Department of Immunology, Mayo Medical and Graduate Schools, Rochester, MinnDorian B McGavern - Scripps Research Institute, La Jolla, CalifDaren R Ure - Department of Immunology, Mayo Medical and Graduate Schools, Rochester, MinnM. Kariuki Njenga - Department of Veterinary Pathobiology at University of Minnesota, MinneapolisAaron J Johnson - Department of Immunology, Mayo Medical and Graduate Schools, Rochester, MinnShunya Nakane - Department of Neurology, Mayo Medical and Graduate Schools, Rochester, MinnMoses Rodriguez - Department of Neurology, Mayo Medical and Graduate Schools, Rochester, Minn
- Publication Details
- Brain pathology (Zurich, Switzerland), Vol.13(3), pp.291-308
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Identifiers
- 99900547658901842
- Language
- English
- Resource Type
- Journal article