Journal article
Direct methods for detection of protein S-nitrosylation
Methods (San Diego, Calif.), Vol.62(2), pp.171-176
08/01/2013
Handle:
https://hdl.handle.net/2376/101268
PMCID: PMC3755045
PMID: 23639867
Abstract
•Recent developments of direct methods for protein SNO labeling are reviewed.•Detailed procedures for the preparation of the biotin-phosphine substrate are reported.•Detailed procedures of using the phosphine substrate to label protein SNO in cells are described.
S-nitrosylation of protein cysteine residues is known to be an important mechanism for nitric oxide signaling. However, the detection of protein S-nitrosylation is still challenging due to technical limitations of current methods. This chapter provides a brief review on recent developments of methods, which directly target S-nitroso moieties for detection. We also describe in detail the protocol of an organophosphine-based biotin labeling of protein S-nitroso moieties.
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Details
- Title
- Direct methods for detection of protein S-nitrosylation
- Creators
- Nelmi O Devarie-Baez - Department of Chemistry, Washington State University, Pullman, WA 99164, USADehui Zhang - Department of Chemistry, Washington State University, Pullman, WA 99164, USASheng Li - Department of Pharmacology & Cancer Biology, Duke University Medical Center, Durham, NC 27710, USAA. Richard Whorton - Department of Pharmacology & Cancer Biology, Duke University Medical Center, Durham, NC 27710, USAMing Xian - Department of Chemistry, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Methods (San Diego, Calif.), Vol.62(2), pp.171-176
- Academic Unit
- Chemistry, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900546572201842
- Language
- English
- Resource Type
- Journal article