Journal article
Direct quantification of fatty acids in wet microalgal and yeast biomass via a rapid in situ fatty acid methyl ester derivatization approach
Applied microbiology and biotechnology, Vol.99(23), pp.10237-10247
12/2015
Handle:
https://hdl.handle.net/2376/108311
PMID: 26276545
Abstract
Accurate determination of fatty acid contents is routinely required in microalgal and yeast biofuel studies. A method of rapid in situ fatty acid methyl ester (FAME) derivatization directly from wet fresh microalgal and yeast biomass was developed in this study. This method does not require prior solvent extraction or dehydration. FAMEs were prepared with a sequential alkaline hydrolysis (15 min at 85 °C) and acidic esterification (15 min at 85 °C) process. The resulting FAMEs were extracted into n-hexane and analyzed using gas chromatography. The effects of each processing parameter (temperature, reaction time, and water content) upon the lipids quantification in the alkaline hydrolysis step were evaluated with a full factorial design. This method could tolerate water content up to 20% (v/v) in total reaction volume, which equaled up to 1.2 mL of water in biomass slurry (with 0.05-25 mg of fatty acid). There were no significant differences in FAME quantification (p>0.05) between the standard AOAC 991.39 method and the proposed wet in situ FAME preparation method. This fatty acid quantification method is applicable to fresh wet biomass of a wide range of microalgae and yeast species.
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Details
- Title
- Direct quantification of fatty acids in wet microalgal and yeast biomass via a rapid in situ fatty acid methyl ester derivatization approach
- Creators
- Tao Dong - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164-6120, USALiang Yu - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164-6120, USADifeng Gao - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164-6120, USAXiaochen Yu - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164-6120, USAChao Miao - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164-6120, USAYubin Zheng - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164-6120, USAJieni Lian - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164-6120, USATingting Li - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164-6120, USAShulin Chen - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164-6120, USA. chens@wsu.edu
- Publication Details
- Applied microbiology and biotechnology, Vol.99(23), pp.10237-10247
- Identifiers
- 99900583052701842
- Language
- English
- Resource Type
- Journal article