Directed mutagenesis confirms the functional importance of positively selected sites in polygalacturonase inhibitor protein

John G Bishop

doi:10.1093/molbev/msi146

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Directed mutagenesis confirms the functional importance of positively selected sites in polygalacturonase inhibitor protein

Journal article

Open access

Peer reviewed

Directed mutagenesis confirms the functional importance of positively selected sites in polygalacturonase inhibitor protein

John G Bishop

Molecular biology and evolution, Vol.22(7), pp.1531-1534

07/2005

DOI: https://doi.org/10.1093/molbev/msi146

Handle:

https://hdl.handle.net/2376/115630

PMID: 15829620

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Abstract

Mutagenesis, Site-Directed

Enzyme Inhibitors - metabolism

Phylogeny

Polygalacturonase - antagonists & inhibitors

Soybeans - genetics

Polygalacturonase - metabolism

Phaseolus - genetics

Plant Proteins - genetics

Point Mutation

Codon

Plant Proteins - chemistry

Enzyme Inhibitors - chemistry

Plant Proteins - metabolism

Polygalacturonase inhibitor proteins (PGIPs) protect plants against invasion by diverse microbial and invertebrate enemies that use polygalacturonase (PG) to breach the plant cell wall. Directed mutagenesis has identified specific natural mutations conferring novel defensive capability in green bean PGIP against a specific fungal PG. These same sites are identified as positively selected by phylogenetic codon-substitution models, demonstrating the utility of such models for connecting retrospective comparative analyses with contemporary, ecologically relevant variation.

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Title: Directed mutagenesis confirms the functional importance of positively selected sites in polygalacturonase inhibitor protein
Creators: John G Bishop
Publication Details: Molecular biology and evolution, Vol.22(7), pp.1531-1534
Academic Unit: Biological Sciences, School of
Publisher: United States
Identifiers: 99900547850701842
Language: English
Resource Type: Journal article