Journal article
Discovery and molecular mapping of a new gene conferring resistance to stem rust, Sr53, derived from Aegilops geniculata and characterization of spontaneous translocation stocks with reduced alien chromatin
Chromosome research, Vol.19(5), pp.669-682
07/2011
Handle:
https://hdl.handle.net/2376/104624
PMID: 21728140
Abstract
This study reports the discovery and molecular mapping of a resistance gene effective against stem rust races RKQQC and TTKSK (Ug99) derived from Aegilops geniculata (2n = 4x = 28, UgUgMgMg). Two populations from the crosses TA5599 (T5DL-5MgL·5MgS)/TA3809 (ph1b mutant in Chinese Spring background) and TA5599/Lakin were developed and used for genetic mapping to identify markers linked to the resistance gene. Further molecular and cytogenetic characterization resulted in the identification of nine spontaneous recombinants with shortened Ae. geniculata segments. Three of the wheat–Ae. geniculata recombinants (U6154-124, U6154-128, and U6200-113) are interstitial translocations (T5DS·5DL-5MgL-5DL), with 20–30% proximal segments of 5MgL translocated to 5DL; the other six are recombinants (T5DL-5MgL·5MgS) have shortened segments of 5MgL with fraction lengths (FL) of 0.32–0.45 compared with FL 0.55 for the 5MgL segment in the original translocation donor, TA5599. Recombinants U6200-64, U6200-117, and U6154-124 carry the stem rust resistance gene Sr53 with the same infection type as TA5599, the resistance gene donor. All recombinants were confirmed to be genetically compensating on the basis of genomic in situ hybridization and molecular marker analysis with chromosome 5D- and 5Mg-specific SSR/STS-PCR markers. These recombinants between wheat and Ae. geniculata will provide another source for wheat stem rust resistance breeding and for physical mapping of the resistance locus and crossover hot spots between wheat chromosome 5D and chromosome 5MgL of Ae. geniculata.
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Details
- Title
- Discovery and molecular mapping of a new gene conferring resistance to stem rust, Sr53, derived from Aegilops geniculata and characterization of spontaneous translocation stocks with reduced alien chromatin
- Creators
- Wenxuan Liu - Wheat Genetic and Genomic Resources Center Department of Plant Pathology Kansas State University Manhattan KS 66506-5502 USAMatthew Rouse - USDA-ARS Cereal Disease Laboratory University of Minnesota St. Paul MN 55108 USABernd Friebe - Wheat Genetic and Genomic Resources Center Department of Plant Pathology Kansas State University Manhattan KS 66506-5502 USAYue Jin - USDA-ARS Cereal Disease Laboratory University of Minnesota St. Paul MN 55108 USABikram Gill - Wheat Genetic and Genomic Resources Center Department of Plant Pathology Kansas State University Manhattan KS 66506-5502 USAMichael Pumphrey - Spring Wheat Breeding and Genetics, Department of Crop and Soil Sciences Washington State University Pullman WA 99164-6420 USA
- Publication Details
- Chromosome research, Vol.19(5), pp.669-682
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 99900547068401842
- Language
- English
- Resource Type
- Journal article