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CC BY V4.0, Open Access
Journal article
Distinct actions of ancestral vinclozolin and juvenile stress on neural gene expression in the male rat
Frontiers in genetics, Vol.6, pp.56-56
2015
Handle:
https://hdl.handle.net/2376/115695
PMCID: PMC4345841
PMID: 25784924
Abstract
Exposure to the endocrine disrupting chemical vinclozolin during gestation of an F0 generation and/or chronic restraint stress during adolescence of the F3 descendants affects behavior, physiology, and gene expression in the brain. Genes related to the networks of growth factors, signaling peptides, and receptors, steroid hormone receptors and enzymes, and epigenetic related factors were measured using quantitative polymerase chain reaction via Taqman low density arrays targeting 48 genes in the central amygdaloid nucleus, medial amygdaloid nucleus, medial preoptic area (mPOA), lateral hypothalamus (LH), and the ventromedial nucleus of the hypothalamus. We found that growth factors are particularly vulnerable to ancestral exposure in the central and medial amygdala; restraint stress during adolescence affected neural growth factors in the medial amygdala. Signaling peptides were affected by both ancestral exposure and stress during adolescence primarily in hypothalamic nuclei. Steroid hormone receptors and enzymes were strongly affected by restraint stress in the mPOA. Epigenetic related genes were affected by stress in the ventromedial nucleus and by both ancestral exposure and stress during adolescence independently in the central amygdala. It is noteworthy that the LH showed no effects of either manipulation. Gene expression is discussed in the context of behavioral and physiological measures previously published.
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Details
- Title
- Distinct actions of ancestral vinclozolin and juvenile stress on neural gene expression in the male rat
- Creators
- Ross Gillette - Institute for Cellular and Molecular Biology, The University of Texas at AustinIsaac Miller-Crews - Institute for Cellular and Molecular Biology, The University of Texas at AustinMichael K Skinner - Center for Reproductive Biology, School of Biological Sciences, Washington State UniversityDavid Crews - Institute for Cellular and Molecular Biology, The University of Texas at Austin
- Publication Details
- Frontiers in genetics, Vol.6, pp.56-56
- Academic Unit
- Biological Sciences, School of
- Publisher
- Frontiers Media S.A
- Number of pages
- 11
- Grant note
- R01 ES023254; R21 ES017538; R01 ES012974 / NIEHS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS) R01ES012974 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
- Identifiers
- 99900547606201842
- Language
- English
- Resource Type
- Journal article