Journal article
Diurnal Variations of Tumor Necrosis Factor Alpha mRNA and Alpha-Tubulin mRNA in Rat Brain
Neuroimmunomodulation, Vol.4(2), pp.84-90
1997
Handle:
https://hdl.handle.net/2376/107483
PMID: 9483199
Abstract
The experiments described herein were designed to determine whether tumor necrosis factor α (TNF-α) displays a diurnal variation in various areas of the normal rat brain. TNF-α mRNA transcripts were detected by reverse-transcriptase polymerase chain reaction. To monitor diurnal changes in TNF-α and α-tubulin expression, rats were sacrificed every 4 h for 24 h starting 1 h after light onset; relative mRNA levels were determined for the cerebellum, cortex, hippocampus, hypothalamus and brainstem. TNF-α mRNA was higher during the light than in the dark phase in the hypothalamus and hippocampus. α-Tubulin mRNA exhibited a similar diurnal variation in the hypothalamus, hippocampus and cortex. In contrast, (β-actin mRNA was lower during the light phase than the dark phase in the hippocampus and cortex. The observed diurnal variations in TNF-α mRNA are consistent with the hypothesis that TNF has a physiological role in the brain.
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Details
- Title
- Diurnal Variations of Tumor Necrosis Factor Alpha mRNA and Alpha-Tubulin mRNA in Rat Brain
- Creators
- Sebastian BredowNandita Guha-ThakurtaPing TaishiFerenc Obál JrJames M Krueger
- Publication Details
- Neuroimmunomodulation, Vol.4(2), pp.84-90
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Basel, Switzerland
- Number of pages
- 7
- Identifiers
- 99900547373501842
- Language
- English
- Resource Type
- Journal article