Diurnal regulation of the gastrin-releasing peptide receptor in the mouse circadian clock

Ilia N Karatsoreos; Russell D Romeo; Bruce S McEwen; Rae Silver

doi:10.1111/j.1460-9568.2006.04633.x

Back

Diurnal regulation of the gastrin-releasing peptide receptor in the mouse circadian clock

Journal article

Open access

Peer reviewed

Diurnal regulation of the gastrin-releasing peptide receptor in the mouse circadian clock

Ilia N Karatsoreos, Russell D Romeo, Bruce S McEwen and Rae Silver

The European journal of neuroscience, Vol.23(4), pp.1047-1053

02/2006

DOI: https://doi.org/10.1111/j.1460-9568.2006.04633.x

Handle:

https://hdl.handle.net/2376/110649

PMCID: PMC3266108

PMID: 16519669

Files and links (1)

url

https://doi.org/10.1111/j.1460-9568.2006.04633.xView

Published (Version of record) Open

Abstract

Blotting, Western - methods

Gastrin-Releasing Peptide - pharmacology

Iodine Isotopes - pharmacokinetics

Mice, Inbred C57BL

Gene Expression Regulation - physiology

Circadian Rhythm - physiology

RNA, Messenger - metabolism

Gastrin-Releasing Peptide - metabolism

Gene Expression Regulation - drug effects

Receptors, Bombesin - metabolism

Animals

Analysis of Variance

Arginine Vasopressin - metabolism

Radioligand Assay - methods

Suprachiasmatic Nucleus - cytology

Mice

Neurons - metabolism

In Vitro Techniques

Neurons - drug effects

In Situ Hybridization - methods

In mammals, circadian rhythms are generated by the suprachiasmatic nuclei (SCN) of the hypothalamus. SCN neurons are heterogeneous and can be classified according to their function, anatomical connections, morphology and/or peptidergic identity. We focus here on gastrin-releasing peptide- (GRP) and on GRP receptor- (GRPr) expressing cells of the SCN. Pharmacological application of GRP in vivo or in vitro can shift the phase of circadian rhythms, and GRPr-deficient mice show blunted photic phase shifting. Given the in vivo and in vitro effects of GRP on circadian behavior and on SCN neuronal activity, we investigated whether the GRPr might be under circadian and/or diurnal control. Using in situ hybridization and autoradiographic receptor binding, we localized the GRPr in the mouse SCN and determined that GRP binding varies with time of day in animals housed in a light-dark cycle but not in conditions of constant darkness. The latter results were confirmed with Western blots of SCN tissue. Together, the present findings reveal that changes in GRPr are light driven and not endogenously organized. Diurnal variation in GRPr activity probably underlies intra-SCN signaling important for entrainment and phase shifting.

Metrics

9 Record Views

See more details

Details

Title: Diurnal regulation of the gastrin-releasing peptide receptor in the mouse circadian clock
Creators: Ilia N Karatsoreos - Department of Psychology, Columbia University, New York, NY 10027, USA
Russell D Romeo
Bruce S McEwen
Rae Silver
Publication Details: The European journal of neuroscience, Vol.23(4), pp.1047-1053
Academic Unit: Integrative Physiology and Neuroscience, Department of
Publisher: France
Grant note: R01 NS037919-02 / NINDS NIH HHS NS37919 / NINDS NIH HHS R01 NS037919 / NINDS NIH HHS
Identifiers: 99900547168201842
Language: English
Resource Type: Journal article