Diversity and evolution of the phenazine biosynthesis pathway

Dmitri V Mavrodi; Tobin L Peever; Olga V Mavrodi; James A Parejko; Jos M Raaijmakers; Philippe Lemanceau; Sylvie Mazurier; Lutz Heide; Wulf Blankenfeldt; David M Weller; Linda S Thomashow

doi:10.1128/AEM.02009-09

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Diversity and evolution of the phenazine biosynthesis pathway

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Diversity and evolution of the phenazine biosynthesis pathway

Dmitri V Mavrodi, Tobin L Peever, Olga V Mavrodi, James A Parejko, Jos M Raaijmakers, Philippe Lemanceau, Sylvie Mazurier, Lutz Heide, Wulf Blankenfeldt, David M Weller, …

Applied and environmental microbiology, Vol.76(3), pp.866-879

02/2010

DOI: https://doi.org/10.1128/AEM.02009-09

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https://hdl.handle.net/2376/106747

PMCID: PMC2813009

PMID: 20008172

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Abstract

Multigene Family

DNA, Bacterial - metabolism

Molecular Sequence Data

Phylogeny

Washington

Biosynthetic Pathways - genetics

Plants - genetics

Base Sequence

Antifungal Agents - metabolism

Escherichia coli - metabolism

Bacteria - classification

Pseudomonas - genetics

Genes, Bacterial

Bacteria - metabolism

Soil Microbiology

Bacterial Proteins - genetics

Genotype

Gene Transfer, Horizontal

Bacteria - genetics

Sequence Analysis, DNA

Plants - metabolism

Sequence Alignment

DNA, Bacterial - genetics

Phenazines - metabolism

Escherichia coli - genetics

Bacterial Proteins - metabolism

Gene Expression Regulation, Bacterial

Pseudomonas - metabolism

Evolution, Molecular

Phenazines are versatile secondary metabolites of bacterial origin that function in biological control of plant pathogens and contribute to the ecological fitness and pathogenicity of the producing strains. In this study, we employed a collection of 94 strains having various geographic, environmental, and clinical origins to study the distribution and evolution of phenazine genes in members of the genera Pseudomonas, Burkholderia, Pectobacterium, Brevibacterium, and Streptomyces. Our results confirmed the diversity of phenazine producers and revealed that most of them appear to be soil-dwelling and/or plant-associated species. Genome analyses and comparisons of phylogenies inferred from sequences of the key phenazine biosynthesis (phzF) and housekeeping (rrs, recA, rpoB, atpD, and gyrB) genes revealed that the evolution and dispersal of phenazine genes are driven by mechanisms ranging from conservation in Pseudomonas spp. to horizontal gene transfer in Burkholderia spp. and Pectobacterium spp. DNA extracted from cereal crop rhizospheres and screened for the presence of phzF contained sequences consistent with the presence of a diverse population of phenazine producers in commercial farm fields located in central Washington state, which provided the first evidence of United States soils enriched in indigenous phenazine-producing bacteria.

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Title: Diversity and evolution of the phenazine biosynthesis pathway
Creators: Dmitri V Mavrodi - Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA. mavrodi@mail.wsu.edu
Tobin L Peever
Olga V Mavrodi
James A Parejko
Jos M Raaijmakers
Philippe Lemanceau
Sylvie Mazurier
Lutz Heide
Wulf Blankenfeldt
David M Weller
Linda S Thomashow
Publication Details: Applied and environmental microbiology, Vol.76(3), pp.866-879
Academic Unit: Plant Pathology, Department of
Publisher: United States
Identifiers: 99900546841201842
Language: English
Resource Type: Journal article