Diversity of thermophilic and non-thermophilic Crenarchaeota at 80 degrees C

Thomas Kvist; Anett Mengewein; Stefanie Manzei; Birgitte K Ahring; Peter Westermann

doi:10.1016/j.femsle.2005.01.021

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Diversity of thermophilic and non-thermophilic Crenarchaeota at 80 degrees C

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Diversity of thermophilic and non-thermophilic Crenarchaeota at 80 degrees C

Thomas Kvist, Anett Mengewein, Stefanie Manzei, Birgitte K Ahring and Peter Westermann

FEMS microbiology letters, Vol.244(1), pp.61-68

03/01/2005

DOI: https://doi.org/10.1016/j.femsle.2005.01.021

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https://hdl.handle.net/2376/109790

PMID: 15727822

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Abstract

DNA, Archaeal - genetics

Fresh Water - microbiology

Temperature

Crenarchaeota - isolation & purification

Gene Library

Genes, Archaeal

In Situ Hybridization, Fluorescence

Hot Temperature

Phylogeny

Genetic Variation

Sulfolobus - genetics

Polymorphism, Restriction Fragment Length

Base Sequence

DNA, Archaeal - isolation & purification

Ecosystem

Cloning, Molecular

RNA, Ribosomal, 16S - genetics

Italy

Sulfolobus - isolation & purification

RNA, Archaeal - genetics

Crenarchaeota - genetics

A hot spring in the solfataric field of Pisciarelli (Naples-Italy) was analysed for Archaeal diversity. Total DNA was extracted from the environment, archaeal 16S rRNA genes were amplified with Archaea specific primers, and a clone library consisting of 201 clones was established. The clones were grouped in 10 different groups each representing a specific band pattern using restriction fragment length polymorphism (RFLP). Members of all 10 groups were sequenced and phylogenetically analyzed. Surprisingly, a high abundance of clones belonging to non-thermophilic Crenarchaeal clusters were detected together with the thermophilic archaeon Acidianus infernus in this thermophilic environment. Neither Sulfolobus species nor other hyperthermophilic Crenarchaeota were detected in the clone library. The relative abundance of the sequenced clones was confirmed by terminal restriction fragment analyses. Amplification of 16S rRNA genes from Archaea transferred from the surrounding environment was considered negligible because DNA from non-thermophilic Crenarchaeota incubated under conditions similar to the solfatara could not be PCR amplified after 5 min.

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Title: Diversity of thermophilic and non-thermophilic Crenarchaeota at 80 degrees C
Creators: Thomas Kvist - Environmental Microbiology & Biotechnology Group, BioCentrum-Danmarks Tekniske Universitet Søltofts Plads, Building 227, DK-2800 Lyngby, Denmark
Anett Mengewein
Stefanie Manzei
Birgitte K Ahring
Peter Westermann
Publication Details: FEMS microbiology letters, Vol.244(1), pp.61-68
Publisher: England
Identifiers: 99900547102601842
Language: English
Resource Type: Journal article