Journal article
Down-regulation followed by re-expression of equine CD4 molecules in response to phorbol myristate acetate
Veterinary immunology and immunopathology, Vol.42(1), pp.71-82
07/1994
Handle:
https://hdl.handle.net/2376/104051
PMID: 7975181
Abstract
The regulatory effects of phorbol myristate acetate (PMA) on the expression of the CD4 molecule on horse T cells were investigated. On both peripheral blood lymphocytes and thymocytes, PMA resulted in a rapid and transient down-regulation of equine CD4 expression, but had no such effect on the surface expression of equine CD5, CD8 or major histocompatibility complex (MHC) class I and class II molecules. Over 75% of the surface CD4 molecules per cell were lost after a 4 h exposure to PMA at 37 degrees C. The regulation of equine CD4 expression induced by PMA was temperature dependent and reversible. The PMA-mediated loss of CD4 expression was inhibited at 4 degrees C. After 24 h of exposure to PMA, CD4 molecules were re-expressed on the cell surface, even in the continued presence of PMA. These findings demonstrate that equine CD4+ T cells undergo alterations in CD4 expression in response to PMA, and suggest that the equine homolog of the CD4 molecule is regulated by PMA in a similar manner to the human CD4 molecule.
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Details
- Title
- Down-regulation followed by re-expression of equine CD4 molecules in response to phorbol myristate acetate
- Creators
- C H Zhang - Cornell Equine Genetics Center, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853G GrünigW DavisD F Antczak
- Publication Details
- Veterinary immunology and immunopathology, Vol.42(1), pp.71-82
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Netherlands
- Grant note
- HD-15799 / NICHD NIH HHS
- Identifiers
- 99900546989501842
- Language
- English
- Resource Type
- Journal article