Dual polarity accurate mass calibration for electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry using maltooligosaccharides

Brian H Clowers; Eric D Dodds; Richard R Seipert; Carlito B Lebrilla

doi:10.1016/j.ab.2008.06.041

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Dual polarity accurate mass calibration for electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry using maltooligosaccharides

Journal article

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Dual polarity accurate mass calibration for electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry using maltooligosaccharides

Brian H Clowers, Eric D Dodds, Richard R Seipert and Carlito B Lebrilla

Analytical biochemistry, Vol.381(2), pp.205-213

2008

DOI: https://doi.org/10.1016/j.ab.2008.06.041

Handle:

https://hdl.handle.net/2376/110709

PMID: 18655765

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Abstract

Mass calibration

Matrix-assisted laser desorption/ionization

Porous-graphitized carbon

Infrared multiphoton dissociation

Electrospray ionization

Oligosaccharide

In view of the fact that memory effects associated with instrument calibration hinder the use of many mass-to-charge ( m/ z) ratios and tuning standards, identification of robust, comprehensive, inexpensive, and memory-free calibration standards is of particular interest to the mass spectrometry community. Glucose and its isomers are known to have a residue mass of 162.05282 Da; therefore, both linear and branched forms of polyhexose oligosaccharides possess well-defined masses, making them ideal candidates for mass calibration. Using a wide range of maltooligosaccharides (MOSs) derived from commercially available beers, ions with m/z ratios from approximately 500 to 2500 Da or more have been observed using Fourier transform ion cyclotron resonance mass spectrometry (FT–ICR–MS) and time-of-flight mass spectrometry (TOF–MS). The MOS mixtures were further characterized using infrared multiphoton dissociation (IRMPD) and nano-liquid chromatography/mass spectrometry (nano-LC/MS). In addition to providing well-defined series of positive and negative calibrant ions using either electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI), the MOSs are not encumbered by memory effects and, thus, are well-suited mass calibration and instrument tuning standards for carbohydrate analysis.

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Title: Dual polarity accurate mass calibration for electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry using maltooligosaccharides
Creators: Brian H Clowers - Department of Chemistry, University of California, Davis, Davis, CA 95616, USA
Eric D Dodds - Department of Chemistry, University of California, Davis, Davis, CA 95616, USA
Richard R Seipert - Department of Chemistry, University of California, Davis, Davis, CA 95616, USA
Carlito B Lebrilla - Department of Chemistry, University of California, Davis, Davis, CA 95616, USA
Publication Details: Analytical biochemistry, Vol.381(2), pp.205-213
Academic Unit: Chemistry, Department of
Publisher: Elsevier Inc
Identifiers: 99900547365001842
Language: English
Resource Type: Journal article