Journal article
Duckweed (Lemna minor) is a novel natural inducer of cellulase production in Trichoderma reesei
Journal of bioscience and bioengineering, Vol.127(4), pp.486-491
04/2019
Handle:
https://hdl.handle.net/2376/117005
PMID: 30366719
Abstract
An inducer is crucial for cellulase production. In this study, duckweed was used as an inducer of cellulase production by Trichoderma reesei RUT C30. In a reaction induced by 50 g/L duckweed in shake flasks, the filter-paper activity (FPA) reached 6.5 FPU/mL, a value comparable to that induced by avicel. The enzyme-hydrolysis rate induced by steam-exploded corn stalk was 54.2%, representing a 28% improvement over that induced by avicel. The duckweed starch was hydrolyzed to glucose, which was subsequently used for biomass accumulation during the fermentation process. Furthermore, to optimize the control of the fermentation process, a combined substrate of avicel and duckweed was used to induce cellulase production by T. reesei RUT C30. The cellulase production and hydrolysis rates of the combined substrate, compared with avicel alone, were 39.6% and 36.7% higher, respectively. The results of this study suggest that duckweed is a good inducer of cellulase production in T. reesei, and it might aid in decreasing the cost of lignocellulosic materials hydrolysis.
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Details
- Title
- Duckweed (Lemna minor) is a novel natural inducer of cellulase production in Trichoderma reesei
- Creators
- Chen Li - Tianjin Key Laboratory of Industrial Biology Systems and Processing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaDemao Li - Tianjin Key Laboratory of Industrial Biology Systems and Processing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaJun Feng - Tianjin Key Laboratory of Industrial Biology Systems and Processing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaXiang Fan - Tianjin Key Laboratory of Industrial Biology Systems and Processing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaShulin Chen - Tianjin Key Laboratory of Industrial Biology Systems and Processing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaDongyuan Zhang - Tianjin Key Laboratory of Industrial Biology Systems and Processing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaRonglin He - Tianjin Key Laboratory of Industrial Biology Systems and Processing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
- Publication Details
- Journal of bioscience and bioengineering, Vol.127(4), pp.486-491
- Publisher
- Elsevier B.V
- Identifiers
- 99900582334201842
- Language
- English
- Resource Type
- Journal article