Dye-doped silica nanoparticle labels/protein microarray for detection of protein biomarkers

Hong Wu; Qisheng Huo; Susan Varnum; Jun Wang; Guodong Liu; Zimin Nie; Jun Liu; Yuehe Lin

doi:10.1039/b719810h

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Dye-doped silica nanoparticle labels/protein microarray for detection of protein biomarkers

Journal article

Open access

Dye-doped silica nanoparticle labels/protein microarray for detection of protein biomarkers

Hong Wu, Qisheng Huo, Susan Varnum, Jun Wang, Guodong Liu, Zimin Nie, Jun Liu and Yuehe Lin

Analyst (London), Vol.133(11), pp.1550-1555

11/2008

DOI: https://doi.org/10.1039/b719810h

Handle:

https://hdl.handle.net/2376/100250

PMCID: PMC2597076

PMID: 18936832

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Abstract

Nanoparticles

Interleukin-6 - analysis

Silicon Dioxide

Humans

Biomarkers - analysis

Immunoassay - methods

Fluorescent Dyes

Nanotechnology

Protein Array Analysis - methods

We report a dye-encapsulated silica nanoparticle as a label, with the advantages of high fluorescence intensity, photostability, and biocompatibility, in conjunction with microarray technology for sensitive immunoassay of a biomarker, interleukin-6 (IL-6), on a microarray format. The tris(2,2'-bipyridyl)ruthenium(ii) chloride hexahydrate (Rubpy) dye was incorporated into silica nanoparticles using a simple one-step microemulsion synthesis. In this synthesis process, Igepal CA520 was used as the surfactant, therefore, no requirement of cosolvent during the synthesis and the particle size was reduced comparing to the commonly used Triton surfactant system. The nanoparticles are uniform in size with a diameter of 50 nm. The microarray fluorescent immunoassay approach based on dye-doped silica nanoparticle labels has high sensitivity for practical applications with a limit of detection for IL-6 down to 0.1 ng mL(-1). The calibration curve is linear over the range from 0.1 ng mL(-1) to 10 ng mL(-1). Furthermore, results illustrated that the assay is highly specific for IL-6 in the presence of range of cytokines or proteins. The RuDS dye-labeled nanoparticles in connection with protein microarrays show the promise for clinical diagnosis of biomarkers.

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Title: Dye-doped silica nanoparticle labels/protein microarray for detection of protein biomarkers
Creators: Hong Wu - Pacific Northwest National Laboratory, Richland, WA 99352, USA
Qisheng Huo
Susan Varnum
Jun Wang
Guodong Liu
Zimin Nie
Jun Liu
Yuehe Lin
Publication Details: Analyst (London), Vol.133(11), pp.1550-1555
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: England
Grant note: U01 NS058161-01 / NINDS NIH HHS U01 NS058161 / NINDS NIH HHS
Identifiers: 99900546693301842
Language: English
Resource Type: Journal article