Journal article
Dynamic changes of substrate reactivity and enzyme adsorption on partially hydrolyzed cellulose
Biotechnology and bioengineering, Vol.114(3), pp.503-515
03/2017
Handle:
https://hdl.handle.net/2376/115533
PMID: 27617791
Abstract
The enzymatic hydrolysis of cellulose is a thermodynamically challenging catalytic process that is influenced by both substrate-related and enzyme-related factors. In this study, a proteolysis approach was applied to recover and clean the partially converted cellulose at the different stages of enzymatic hydrolysis to monitor the hydrolysis rate as a function of substrate reactivity/accessibility and investigate surface characteristics of the partially converted cellulose. Enzyme-substrate interactions between individual key cellulase components from wild-type Trichoderma reesei and partially converted cellulose were followed and correlated to the enzyme adsorption capacity and dynamic sugar release. Results suggest that cellobiohydrolase CBH1 (Cel7A) and endoglucanases EG2 (Cel5A) adsorption capacities decreased as cellulose was progressively hydrolyzed, likely due to the "depletion" of binding sites. Furthermore, the degree of synergism between CBH1 and EG2 varied depending on the enzyme loading and the substrates. The results provide a better understanding of the relationship between dynamic change of substrate features and the functionality of various cellulase components during enzymatic hydrolysis. Biotechnol. Bioeng. 2017;114: 503-515. © 2016 Wiley Periodicals, Inc.
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Details
- Title
- Dynamic changes of substrate reactivity and enzyme adsorption on partially hydrolyzed cellulose
- Creators
- Jian Shi - Department of Biosystems and Agricultural Engineering, University of Kentucky, Lexington, KentuckyDong Wu - Biological and Materials Science Center, Sandia National Laboratories, Livermore, CaliforniaLibing Zhang - Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, Richland, WashingtonBlake A Simmons - Deconstruction Division, Joint BioEnergy Institute, Emeryville, CaliforniaSeema Singh - Biological and Materials Science Center, Sandia National Laboratories, Livermore, CaliforniaBin Yang - Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, Richland, WashingtonCharles E Wyman - BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee
- Publication Details
- Biotechnology and bioengineering, Vol.114(3), pp.503-515
- Academic Unit
- Biological Systems Engineering, Department of; Chemical Engineering and Bioengineering, School of
- Publisher
- United States
- Identifiers
- 99900547771201842
- Language
- English
- Resource Type
- Journal article