Journal article
Dynorphin-mediated antinociceptive effects of l-arginine and SIN-1 (an NO donor) in mice
Brain research bulletin, Vol.70(3), pp.245-250
2006
Handle:
https://hdl.handle.net/2376/110402
PMID: 16861110
Abstract
The antinociceptive response of mice to the amino acid
l-arginine (
l-ARG) has been attributed to either an opioid mechanism or a non-opioid but nitric oxide (NO)-dependent mechanism. Earlier it was reported that the mechanism of nitrous oxide-induced antinociception involved opioid components and was also dependent on brain NO. This study was designed to determine whether the antinociceptive effects of
l-ARG and the NO donor 3-morpholinosydnoimine (SIN-1) might be mediated by brain mechanisms similar to those that are responsible for nitrous oxide (N
2O) antinociception.
l-ARG and SIN-1 were administered to mice intracerebroventricularly (i.c.v.), and antinociception was assessed using the acetic acid abdominal constriction test. Both
l-ARG and SIN-1 caused dose-related antinociceptive effects that were blocked by naloxone and norbinaltorphimine. The antinociceptive effects of both SIN-1 and
l-ARG were also blocked to a greater extent by i.c.v. administration of a rabbit antiserum against rat dynorphin 1–13 than an antiserum against methionine-enkephalin, suggesting that the SIN-1 and
l-ARG effects may be related to stimulated release of dynorphin. The antinociceptive effect of
l-ARG was antagonized by an inhibitor of neuoronal NO synthase enzyme, indicating that
l-ARG had to be converted to NO for its antinociceptive action. These findings indicate that the mechanisms of antinociceptive action of
l-ARG and SIN-1 are both mediated by dynorphin and dependent on NO.
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Details
- Title
- Dynorphin-mediated antinociceptive effects of l-arginine and SIN-1 (an NO donor) in mice
- Creators
- Eunhee Chung - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USABrianna Burke - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USAAndrew J Bieber - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USAJason C Doss - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USAYusuke Ohgami - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USARaymond M Quock - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USA
- Publication Details
- Brain research bulletin, Vol.70(3), pp.245-250
- Academic Unit
- Psychology, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547191501842
- Language
- English
- Resource Type
- Journal article